| HPLC法测定人血浆中奥卡西平活性代谢产物单羟基卡马西平的浓度 |
| 投稿时间：2020-05-14 修订日期：2020-07-10 点此下载全文 |
| 引用本文：姚囡囡,周宇,付文倩,黄珊,宋洪涛.HPLC法测定人血浆中奥卡西平活性代谢产物单羟基卡马西平的浓度[J].药学实践杂志,2021,39(1):53~57 |
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| 基金项目:中国人民解放军联勤保障部队第九〇〇医院院内课题（2019L16） |
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| 中文摘要:目的 建立HPLC法测定奥卡西平活性代谢产物单羟基卡马西平的血药浓度。方法 血浆样品经甲醇处理后，以奥硝唑为内标，采用HPLC法测定，其中，色谱柱为ZORBAX Eclipse XDB-C18（150 mm×4.6 mm，5 μm），流动相为水-乙腈（80∶20，V/V），流速1.0 ml/min，柱温35 ℃，双波长检测：192 nm检测单羟基卡马西平，318 nm检测奥硝唑。结果 单羟基卡马西平血药浓度在2～50 μg/ml（r=0.998 6）范围内线性关系良好，定量下限浓度为2 μg/ml；准确度在95.57%～100.59%之间，日内、日间精密度RSD均小于15%；单羟基卡马西平及内标的平均提取回收率在89.62%～98.76%之间；稳定性的RSD均小于6%。结论 该方法专属性强、灵敏度高，且操作简便，可用于单羟基卡马西平血药浓度的临床检测。 |
| 中文关键词:奥卡西平 单羟基卡马西平 高效液相色谱 血药浓度 |
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| The assay of monohydroxy carbamazepine, the active metabolite of oxcarbazepine, in human plasma by HPLC |
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| Abstract:Objective To establish an assay method for monohydroxy carbamazepine, the active metabolite of oxcarbazepine, in human plasma.Methods Ornidazole was used as the internal standard. Plasma samples were processed with methanol and analyzed by HPLC. The column was ZORBAX Eclipse XDB-C18(150 mm×4.6 mm, 5 μm) with the mobile phase of water-acetonitrile (80∶20, V/V) at a flow rate of 1.0 ml/min. Dual wavelength detection is applied. The detection wavelength of monohydroxy carbamazepine was set at 192 nm and ornidazole at 318 nm.Results There was an excellent liner relationship for monohydroxy carbamazepine from 2 to 50 μg/ml(r= 0.998 6). The limit of quantification was 2 μg/ml with the range of accuracy between 95.57% and 100.59%. The RSD of intra-day and inter-day precisions were less than 15%. The average extraction recovery rate of MHC and internal standard were in the range of 89.62% to 98.76%. The RSD of stability was less than 6%.Conclusion This method is specific, sensitive, and easy to operate. It is suitable for the clinical assay of monohydroxy carbamazepine in human plasma. |
| keywords:oxcarbazepine monohydroxy carbazepine HPLC plasma concentration |
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