| RP-HPLC法同时测定防暑清热饮中5种活性成分的含量 |
| 投稿时间：2020-02-20 修订日期：2020-04-03 点此下载全文 |
| 引用本文：李杰,杨育儒,王庆芬,陈锦珊.RP-HPLC法同时测定防暑清热饮中5种活性成分的含量[J].药学实践杂志,2020,38(5):462~465 |
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| 基金项目:医院青年苗圃基金(17Y019) |
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| 中文摘要:目的 建立RP-HPLC法同时测定防暑清热饮中绿原酸、木犀草素-7-O-β-D-葡糖苷、3，5-O-二咖啡酰奎宁酸、蒙花苷和广藿香酮含量的方法。方法 采用ZORBAX-SB-C18色谱柱(250 mm×4.6 mm,5 μm)，以0.2%磷酸溶液（A）-乙腈（B）梯度洗脱，流速为1.0 ml/min，检测波长为327 nm，柱温为30 ℃。结果 绿原酸、木犀草素-7-O-β-D-葡糖苷、3，5-O-二咖啡酰奎宁酸、蒙花苷和广藿香酮线性关系良好（r≥0.999 6），平均加样回收率（RSD）分别为102.03%（1.63%）、102.38%（1.51%）、102.39%（1.23%）、103.14%（1.87%）和104.01%（2.33%）。结论 本实验建立的测定方法简单、准确、重复性好，能够有效控制该制剂的质量。 |
| 中文关键词:防暑清热饮 绿原酸 木犀草素-7-O-β-D-葡糖苷 3，5-O-二咖啡酰奎宁酸 蒙花苷 广藿香酮 高效液相色谱法 |
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| Simultaneous determination of five active components in Fangshu Qingre mixture by RP-HPLC |
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| Abstract:Objective To establish a method for simultaneous determination of chlorogenic acid, luteolin-7-O-β-D-glucoside,3,5-dicaffeoylquinic acid, linarin and pogostone in Fangshu Qingre mixture by RP-HPLC.Methods ZORBAX-SB-C18 column(4.6 mm×250 mm, 5 μm) was used as the chromatographic column.The mobile phase was 0.2% formic acid water solution(A) and acetonitrile solution (B)with a gradient elution mode. The flow rate was 1.0 ml/min.The detective wavelength was 327 nm. The column temperature was 30 ℃.Results There were good linear relationships in the determination of chlorogenic acid, luteolin-7-O-β-D-glucoside, 3,5-dicaffeoylquinic acid, linarin and pogostone (r≥0.999 6), with the average recovery rate of 102.03%(1.63%), 102.38%(1.51%), 102.39%(1.23%), 103.14%(1.87%) and 104.01%(2.33%).Conclusion The method was simple and stable with a good reproducibility, which could be used as a quality control method for active compotents in Fangshu Qingre mixture. |
| keywords:Fangshu Qingre mixture chlorogenic acid luteolin-7-O-β-D-glucoside 3,5-dicaffeoylquinic acid linarin pogostone HPLC |
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