| 4个黄芪异黄酮类化合物对PC 12细胞分化的影响 |
| 投稿时间：2020-01-10 修订日期：2020-03-06 点此下载全文 |
| 引用本文：吴丘玲,成彦琼,刘爱军,张卫东.4个黄芪异黄酮类化合物对PC 12细胞分化的影响[J].药学实践杂志,2020,38(3):232~236 |
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| 中文摘要:目的 探究毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷对PC 12细胞分化的影响。方法 培养PC 12细胞，与不同浓度的神经生长因子（NGF）、毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷共处理5 d，1次/d，连续3次，观察PC 12细胞突起向外生长的情况。同时，用免疫荧光染色检测上述化合物对β微管蛋白（β III-tubulin）表达的影响。结果 与对照组相比，毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷和芒柄花苷（0.01~10.00 μmol/L）未能促进PC 12细胞突起向外生长和β微管蛋白的表达。结论 毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷不能促进PC 12细胞分化。 |
| 中文关键词:毛蕊异黄酮 刺芒柄花素 毛蕊异黄酮苷 芒柄花苷 PC 12细胞 细胞分化 |
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| The effect of four compounds of astragalus isoflavones on the differentiation of PC 12 cells |
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| Abstract:Objective To investigate the effects of calycosin, formononetin, calycosin-7-glucoside and ononin on PC 12 cells differentiation.Methods PC 12 cells were cultured and treated with different concentrations of nerve growth factor (NGF), calycosin, formononetin, calycosin-7-glucoside and ononin for 5 days, once a day, 3 times in a row. The neurite outgrowth of PC 12 cells was observed and the expression of β III-tubulin were measured by immunofluorescence.Results Compared with the vehicle group, neurite outgrowth and the expression of β III tubulin in PC 12 cells had not promoted by calycosin, formononetin, calycosin-7-glucoside and ononin (0.01-10.00 μmol/L).Conclusion PC 12 cells differentiation could not be induced by calycosin, formononetin, calycosin-7-glucoside and ononin. |
| keywords:calycosin formononetin calycosin-7-glucoside ononin PC 12 cells cell differentiation |
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