| 表面增强拉曼光谱对结构类似物黄嘌呤、茶碱、可可碱的鉴别 |
| 投稿时间：2020-01-03 修订日期：2020-02-24 点此下载全文 |
| 引用本文：崔晓林,陆峰.表面增强拉曼光谱对结构类似物黄嘌呤、茶碱、可可碱的鉴别[J].药学实践杂志,2020,38(3):227~231,258 |
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| 基金项目:国家重大新药创制科技重大专项（2018ZX09J18112） |
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| 中文摘要:目的 采用表面增强拉曼光谱技术对结构类似物黄嘌呤、茶碱、可可碱进行区分。方法 通过制备浓缩的银胶增强试剂作为拉曼基底，增加单位面积内的“热点”数目，从而提高表面增强拉曼光谱的灵敏度，增强待测样品的信号强度，实现对结构类似物进行有效区分的目的。同时，通过测定包含3种混合物的血清样品，验证表面增强拉曼光谱在实际应用中的可行性。结果 浓缩后的银胶极大地提高了3种结构类似物的信号强度，分别得到3种物质各自的特征光谱图，以及混合物在血清体系下的光谱图。3种物质水溶液的检测限依次为：0.005、0.01、0.005 μmol/L。结论 表面增强拉曼光谱是一种很好的用于区分结构类似物的分析方法，具有简便快捷、灵敏度高、对样品无损等特点，可广泛应用于检测、分析、临床治疗和诊断等领域。 |
| 中文关键词:表面增强拉曼散射光谱 黄嘌呤 茶碱 可可碱 鉴别 |
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| Identification of structural analogues xanthine, theophylline and theobromine by surface-enhanced Raman spectroscopy |
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| Abstract:Objective To distinguish the structural analogues xanthine, theophylline and theobromine by surface-enhanced Raman spectroscopy.Methods Concentrated silver colloid enhancement reagent was prepared as the Raman substrate to increase the number of "hot spots" per unit area, improve the sensitivity of surface-enhanced Raman spectroscopy, enhance the signal strength of the samples and achieve the effective discrimination of structural analogues. Meanwhile, the feasibility of surface-enhanced Raman spectroscopy in practical application was verified by determining serum samples of three mixtures.Results The concentrated silver colloid greatly increased the Raman intensity of the three structural analogues. The spectra of each individual compound and the mixture in the serum system was obtained. The detection limit of the three substances in aqueous solution were 0.005, 0.01 and 0.005 μmol/L respectively.Conclusion Surface-enhanced Raman spectroscopy is a potent technique for distinguishing structural analogues. It is rapid, sensitive and nondestructive to samples. Hence, it can be widely used in the fields of detection, analysis, clinical treatment and diagnosis. |
| keywords:surface-enhanced Raman spectroscopy xanthine theophylline theobromine distinguish |
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