| 豆豉姜活性成分9,9’-O-二-(E)-阿魏酰基-内消旋-5,5’-二甲氧基开环异落叶松树脂酚（LCA）抗炎作用研究 |
| 投稿时间：2019-12-30 修订日期：2020-01-11 点此下载全文 |
| 引用本文：余璐瑶,贾丹,沈慧,孙晓蕾,秦路平,韩婷.豆豉姜活性成分9,9’-O-二-(E)-阿魏酰基-内消旋-5,5’-二甲氧基开环异落叶松树脂酚（LCA）抗炎作用研究[J].药学实践杂志,2020,38(3):216~220 |
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| 基金项目:国家自然科学基金（81573696） |
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| 中文摘要:目的 对傣药豆豉姜活性成分LCA在动物及细胞水平上的抗炎作用进行评价。方法 采用耳肿胀和棉球肉芽肿模型进行LCA抗炎的体内药效学评价。采用脂多糖（LPS）诱导的小鼠单核巨噬细胞（RAW264.7），通过检测其一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）的分泌量以及诱导型一氧化氮合酶（iNOS）、环氧合酶-2（COX-2）蛋白的表达水平，以评价LCA的体外抗炎活性。结果 在小鼠耳肿胀的实验中，LCA组的耳肿胀度明显低于模型组，且高浓度组对小鼠耳肿胀的抑制率高于阳性药组；在大鼠棉球肉芽肿实验中，LCA高剂量组显著减轻了棉球肉芽肿的湿重与干重。此外，LCA显著减少LPS诱导的RAW264.7细胞中NO、TNF-α的分泌，降低iNOS、COX-2蛋白的表达。结论 LCA具有显著的体内外抗炎作用，值得进一步深入研究。 |
| 中文关键词:LCA 抗炎 耳肿胀 肉芽肿 RAW264.7细胞 |
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| Anti-inflammatory study on 9,9'-O-di-(E）-feruloyl-meso-5,5'-dimethoxysecoisolariciresinol (LCA), an active ingredient in Litsea cubeba (Lour.) Pers |
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| Abstract:Objective To evaluate the anti-inflammatory effects of LCA, which is the active component from Litsea cubeba (Lour.) Pers.Methods Pharmacodynamic evaluations of ear swelling and cotton ball granuloma models were used in animal experiments. In vitro experiment, lipopolysaccharide (LPS) stimulated monocyte macrophage RAW264.7 cells were used to evaluate the anti-inflammatory activity of LCA by detecting the secretions of nitric oxide (NO), tumor necrosis factor-α (TNF-α), the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein.Results In ear swelling experiment, the extent of ear swelling was significantly lower in the LCA treated group than the model group. The inhibition rate was greater in the high LCA concentration group than the positive drug group. In addition, LCA reduced the weight of cotton ball granuloma markedly. At the cell level, LCA significantly inhibited the secretions of NO, TNF-α and reduced the expressions of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells.Conclusion LCA has significant anti-inflammatory effects in vitro and in vivo. The further studies are warranted for the development of anti-inflammatory drugs. |
| keywords:LCA anti-inflammatory ear swelling granuloma of cotton ball RAW264.7 cell |
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