| 基于抗氧化和抗炎生物效应的生脉注射液质量评价 |
| 投稿时间：2019-06-12 修订日期：2019-11-20 点此下载全文 |
| 引用本文：俞月,路娟,柴瑞平,吕欣锴,邓明慧,陈曦.基于抗氧化和抗炎生物效应的生脉注射液质量评价[J].药学实践杂志,2020,38(2):143~147 |
| 摘要点击次数: 1346 |
| 全文下载次数: 1212 |
|
| 基金项目:国家自然科学基金面上项目（No.81673667）；中国医学科学院医学与健康科技创新工程经费资助（No.2016-I2M-3-015, No.2017-I2M-B&R-09） |
|
| 中文摘要:目的 建立以抗氧化和抗炎效应为指标的生脉注射液质量评价方法。方法 以水溶性维生素E(Trolox)和N-硝基-L-精氨酸甲酯(L-NAME)作为对照药，采用三硝基苯肼(DPPH)法和一氧化氮(NO)检测试剂盒对其抗氧化效应和对脂多糖(LPS)诱导单核巨噬细胞RAW264.7释放NO的抑制能力进行测定，将样品换算为相应浓度的对照药，对其方法学和生物效应进行控制和评价。结果 各批次样品均具有较好的抗炎和抗氧化效应，精密度高，重复性好；各批次样品在抗氧化能力方面不存在较大差异，在抗炎能力方面样品2和样品8与其他样品存在较大差异。结论 建立的方法可以准确评价生脉注射液的抗氧化和抗炎效应。 |
| 中文关键词:生脉注射液 生物效应 质量控制 抗氧化 抗炎 |
| |
| Quality control and evaluation of Shengmai injection based on anti-oxidant and anti-inflammatory biological effects |
|
|
| Abstract:Objective To establish a quality evaluation method for Shengmai injection with antioxidant and anti-inflammatory effects as indicators.Methods Using water-soluble vitamin E (Trolox) and N-nitro-L-arginine methyl ester (L-NAME) as control drugs, using trinitrophenyl-hydrazine (DPPH) method and nitric oxide (NO) detection kit, the anti-oxidation effect and the inhibition ability of lipopolysaccharide (LPS)-induced NO release from mononuclear macrophage RAW264.7 were measured. The sample was converted into the corresponding concentration of the control drug to control and evaluate its methodological and biological effects.Results Each batch of samples had good anti-inflammatory and anti-oxidation effects with high precision and good repeatability. There was no significant difference in the anti-inflammation capacity among batches of. Samples 2 and 8 were significant differences from other samples in anti-inflammatory ability.Conclusion The established method can accurately evaluate the anti-oxidant and anti- inflammatory effects of Shengmai injection. |
| keywords:Shengmai injection biological effect quality control anti-oxidant anti-inflammatory |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
