| 医用透明质酸钠凝胶对肿瘤生长和转移影响的实验研究 |
| 投稿时间：2019-11-26 修订日期：2020-01-09 点此下载全文 |
| 引用本文：蔡同凯,杨文胜,曹永兵,韩华,阎澜.医用透明质酸钠凝胶对肿瘤生长和转移影响的实验研究[J].药学实践杂志,2020,38(2):129~134 |
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| 中文摘要:目的 考察医用透明质酸钠凝胶（medical sodium hyaluronate gel，HA）在体外和裸鼠体内对腹、盆腔相关肿瘤生长和转移的影响。方法 利用Hela、CT26和HCT116等3株肿瘤细胞，通过MTT法和Transwell实验分别考察不同浓度HA体外对肿瘤细胞生长和迁移的影响；建立裸鼠结肠原位种植瘤模型，比较瘤块接种4周后不同实验组的瘤块体积和瘤重，考察HA体内对结肠癌HCT116增殖的影响；通过比较接种3周后不同实验组裸鼠的肺和肝脏的肿瘤转移率和转移病灶数，考察HA对结肠癌CT26体内转移的影响。结果 不同浓度HA在体外均未促进Hela、CT26和HCT116细胞的生长和转移，且在5 mg/ml HA浓度下，显示出一定的抑制作用。体内结果显示，HA未促进结肠癌HCT116肿瘤的生长，却显示出明显抑制CT26肿瘤的转移。结论 在本实验条件下，HA在体内、外均未促进腹、盆腔相关肿瘤细胞Hela、CT26和HCT116的生长、迁移和转移。 |
| 中文关键词:透明质酸钠 肿瘤生长 肿瘤转移 原位种植瘤 |
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| The effects of medical sodium hyaluronate gel on the tumor proliferation and metastasis |
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| Abstract:Objective To investigate the effect of medical sodium hyaluronate gel (HA) on the growth and metastasis of abdominal and pelvic tumor cells in vitro and in nude mice.Methods Three tumor cells, Hela, CT26 and HCT116, were used to investigate the effects of different HA concentrations on the growth and migration of tumor cells in vitro by MTT assay and Transwell assay. An orthotopic transplantation model of colonic tumor in nude mice was established to investigate the effect on the proliferation of cell HCT116 by comparing the tumor volume and tumor mass 4 weeks after inoculation. The effects on the metastasis of cell CT26 were investigated by comparing the tumor metastasis rate and the number of metastatic lesions of lung and liver in nude mice among the different experimental groups 3 weeks after inoculation.Results HA did not promote the growth and metastasis of Hela, CT26 and HCT116 cells in vitro at different concentrations. Actually, HA exhibited a certain inhibitory activity at the concentration of 5 mg/ml. In the orthotopic transplantation model of colonic tumor-HCT116, HA did not promote the growth of cell HCT116. In the orthotopic transplantation model of colonic tumor-CT26, HA inhibited CT26 tumor metastasis.Conclusion Under the experimental conditions, HA did not promote the growth, migration or metastasis of abdominal and pelvic related tumor cells including Hela, CT26 and HCT116 in vitro and in vivo. |
| keywords:Medical sodium hyaluronate tumor growth tumor metastasis orthotopic transplantation tumor |
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