| 头孢拉定微透析体外回收率及影响因素的研究 |
| 投稿时间：2019-10-15 修订日期：2019-12-27 点此下载全文 |
| 引用本文：仰先蜜,许小建,王学彬,王卓,王桐生.头孢拉定微透析体外回收率及影响因素的研究[J].药学实践杂志,2020,38(2):120~123,128 |
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| 基金项目:国家自然科学基金（81870520） |
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| 中文摘要:目的 测定头孢拉定微透析体外回收率及影响因素。方法 采用微透析浓度差法（减量法、增量法）和液质联用技术（LC-MS/MS）测定头孢拉定的体外回收率，并考察流速、浓度对回收率的影响，以探讨微透析技术用于头孢拉定体内药动学研究的可行性。结果 所建立的方法在要求范围内线性关系良好，方法灵敏可靠。增、减量法测得的回收率无显著性差异。相同条件下，探针体外回收率随流速增大而减小，不受探针周围药物浓度的影响。结论 微透析技术可用于头孢拉定药动学研究，减量法可用于头孢拉定微透析体内回收率和药动学参数的测定。 |
| 中文关键词:微透析 头孢拉定 体外回收率 |
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| Microdialysis recovery of cefradine in vitro |
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| Abstract:Objective To determine the in vitro recovery rate and influencing factors of cefradine microdialysis.Methods Two different methods (loss method, gain method) of microdialysis concentration and LC-MS/MS were used to determine the in vitro recovery rate of cefradine. The effect of flow rate and concentration of the perfusate on the recovery rate were investigated. To explore the feasibility of microdialysis technology for pharmacokinetic studies in cefradine.Results The LC-MS/MS analysis method was linear in the required range and the method was sensitive and reliable. There was no significant difference in the recovery rate measured by gain or loss method. Under the same conditions, the in vitro recovery of the probe decreased with increasing flow rate, independent of the drug concentration around the probe.Conclusion Microdialysis technique could be used to study the pharmacokinetics of cefradine, and loss method could be used to determine the in vivo recovery rate and pharmacokinetics of cefradine on microdialysis. |
| keywords:microdialysis cefradine in vitro recovery |
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