| 二氯乙酸钠对氧糖剥夺损伤的BV2细胞的保护作用及其机制研究 |
| 投稿时间：2018-10-20 修订日期：2018-12-27 点此下载全文 |
| 引用本文：赵辉,章越凡,李铁军.二氯乙酸钠对氧糖剥夺损伤的BV2细胞的保护作用及其机制研究[J].药学实践杂志,2019,37(2):146~150 |
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| 基金项目:浦东新区科技发展基金（PKJ2015-Y26）；浦东新区卫生和计划生育委员会学科建设项目（PWZbr 2017-16） |
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| 中文摘要:目的 研究二氯乙酸钠（dichloroacetate，DCA）对氧糖剥夺（oxygen glucose deprivation，OGD）损伤模型中小鼠小胶质细胞（BV2细胞）的保护作用，并探讨其作用机制。方法 将BV2细胞分为3组：对照组、OGD组、DCA治疗组，通过OGD 4 h建立损伤模型。CCK-8和流式细胞仪检测细胞凋亡及ROS和NO的表达，Western blot检测NF-κB通路相关蛋白表达水平。结果 CCK-8及流式细胞检测结果表明，DCA可显著降低OGD诱导的BV2细胞凋亡，并且减少OGD后细胞中活性氧（ROS）和一氧化氮（NO）的表达（P<0.05）。Western blot结果显示，DCA可显著影响OGD损伤介导的BV2细胞JNK、I-κB和NF-κB蛋白表达水平（P<0.05）。结论 DCA对OGD损伤的BV2细胞具有保护作用，其机制与抗凋亡、抗氧化和抗炎作用有关。 |
| 中文关键词:二氯乙酸钠 氧糖剥夺 ROS NF-κB |
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| Protective effect and mechanism of sodium dichloroacetate on BV2 cells damaged by OGD |
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| Abstract:Objective To study the protective effect and mechanism of dichloroacetate (DCA) on BV2 microglia in oxygen glucose deprivation (OGD) injury model.Methods BV2 cells were divided into 3 groups:CON group,OGD group and DCA intervention group.The injury model was established by OGD 4 h.CCK-8 and flow cytometry was used to detect apoptosis and expression of ROS and NO in cells.Western blot was used to detect the expression of proteins related to NF-κB pathway.Results CCK-8 and flow cytometry showed that the apoptosis of BV2 cells induced by OGD and the expression of ROS and NO in OGD cells were decreased significantly by DCA (P<0.05).Western blot analysis showed that the expression of I-κB,JNK and NF-κB protein in BV2 cells mediated by OGD injury were influenced significantly by DCA (P<0.05).Conclusion DCA had a protective effect on OGD-injured BV2 cells through anti-oxidation and anti-apoptosis. |
| keywords:dichloroacetate OGD ROS NF-κB |
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