| HPLC法测定麻杏抗感颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量 |
| 投稿时间：2017-11-23 修订日期：2018-04-08 点此下载全文 |
| 引用本文：郝哲,王丽军,张建帮.HPLC法测定麻杏抗感颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量[J].药学实践杂志,2018,36(4):355~357 |
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| 基金项目:军队医疗机构制剂标准提高科研专项课题重点项目（14ZJZ02） |
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| 中文摘要:目的 建立麻杏抗感颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量测定方法。方法 采用高效液相色谱（HPLC）法，色谱柱：Wondasil C18（4.6 mm×250 mm，5 μm）；流动相：乙腈-0.1%磷酸溶液（5：95）；流速：1 ml/min；检测波长：215 nm；柱温：30℃；进样量：10 μl。结果 盐酸麻黄碱、盐酸伪麻黄碱的质量浓度分别在4.231~42.31 μg/ml（r=0.999 7）、1.187~11.87 μg/ml（r=0.999 9）范围内与各自峰面积线性关系良好，平均加样回收率为分别为98.9%、98.1%；RSD分别为0.48%、0.64%。结论 该法准确度高、重复性好， 可用于麻杏抗感颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量测定。 |
| 中文关键词:麻杏抗感颗粒 盐酸麻黄碱 盐酸伪麻黄碱 |
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| Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules by HPLC |
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| Abstract:Objective To establish a method of HPLC for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules. Methods Wondasil C18(4.6 mm×250 mm,5 μm)was used at 30℃ and the acetonitrile-0.1% H3PO4 (5:95) was the mobile phase with a flow rate of 1.0 ml/min. The detection wavelength was at 215nm. Results The linear range of ephedrine hydrochloride and pseudoephedrine hydrochloride were 4.231-42.31 μg/ml(r=0.999 7)、1.187-11.87 μg/ml(r=0.999 9), the average recovery were 98.9% (RSD=0.48%,n=6) and 98.1%(RSD=0.48%,n=6). Conclusion This method was accurate and good reproducibility, which could be used for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Maxing Kanggan Granules. |
| keywords:Maxing Kanggan Granules ephedrine hydrochloride pseudoephedrine hydrochloride |
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