| 高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物 |
| 投稿时间：2017-10-02 修订日期：2018-03-26 点此下载全文 |
| 引用本文：张圳,冯家懿,果卉,姚媛,闻俊,周婷婷.高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物[J].药学实践杂志,2018,36(4):347~350 |
| 摘要点击次数: 1139 |
| 全文下载次数: 967 |
| 作者 | 单位 | E-mail | | 张圳 | 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433 | | | 冯家懿 | 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433 | | | 果卉 | 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433 | | | 姚媛 | 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433
安徽中医药大学药学院, 安徽 合肥 230012 | | | 闻俊 | 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433 | | | 周婷婷 | 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433 | tingting_zoo@163.com |
|
| 基金项目:国家自然科学基金资助项目（81573584，81773862）；第二军医大学本科生创新孵化基地项目（FM2016071） |
|
| 中文摘要:目的 建立HPLC法测定体外大鼠肠道菌液中的大豆苷及其代谢物，研究大鼠肠道菌群对大豆苷的代谢情况。方法 采用大鼠离体粪便温孵法，将大豆苷与大鼠肠道菌群培养液进行厌氧温孵。温孵样品采用甲醇沉淀蛋白处理后进行HPLC法分析，色谱条件：色谱柱为Diamonsil C18柱；流动相为0.2%乙酸水溶液与甲醇，梯度洗脱；检测波长为260 nm；柱温为25℃；进样量为10 μl。结果 大豆苷及其代谢物大豆苷元分别在98.0~490、57.6~288 μg/ml浓度范围内线性良好，高、中、低3个浓度的相对回收率在96.6%~114.0%之间，相对标准偏差小于9.9%，表明本法的准确度和精密度符合要求。大豆苷在肠道菌液中代谢生成大豆苷元，其消除半衰期为37.6 min。结论 所建立的HPLC法适合同时测定体外大鼠肠道菌液中的大豆苷及其代谢物，大豆苷在体外代谢的主要途径为糖苷键断裂转化成对应苷元。 |
| 中文关键词:肠道菌群 体外代谢 大豆苷 高效液相色谱法 |
| |
| Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC |
|
|
| Abstract:Objective To establish an HPLC method for simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro, and study the metabolism of daidzin by rat intestinal bacteria. Methods The incubation of daidzin was carried out at 37℃ with rat intestinal bacteria test solution. The samples were precipitated by methanol followed by a separation with HPLC method. The conditions were as follows:the chromatographic column was Ultimate C18; the mobile phase was 0.2% acetic acid and methanol (gradient elution); the detection wavelength was 260nm, and the column temperature was 25℃. Results Daidzin and daidzein showed good linearity with the range of 98.0~490μg/ml and 57.6~288μg/ml, respectively. The accuracy of daidzin and daidzein was ranged from 96.6%~114.0% and the precision of the two analytes was within 9.9%. Daidzin was transformed to daidzein in the rat intestinal bacteria test solution, and its elimination half-life was 37.6 min. Conclusion The established HPLC method was suitable for the determination of daidzin and its metabolite. Daidzin could be metabolized by rat intestinal bacteria in vitro,and the main metabolic pathway was transforming daidzin into corresponding aglycone through breaking the glucoside bond |
| keywords:intestinal bacteria metabolism in vitro daidzin HPLC |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
