| 清脂胃舒佐餐茶HPLC法指纹图谱的研究及4个成分的含量测定 |
| 投稿时间：2017-11-09 修订日期：2018-03-26 点此下载全文 |
| 引用本文：肖会敏,何悦,雷美娜,王四旺.清脂胃舒佐餐茶HPLC法指纹图谱的研究及4个成分的含量测定[J].药学实践杂志,2018,36(4):324~328 |
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| 基金项目:陕西省创新药物研究中心（现代中药新药研发）平台建设（2015SF2-08-01） |
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| 中文摘要:目的 建立清脂胃舒佐餐茶水溶性成分的高效液相色谱（HPLC）法指纹图谱，并测定没食子酸、绿原酸、柚皮苷、橙皮苷的含量。方法 采用HPLC法，色谱条件：Kromasil C18柱（250 mm×4.6 mm， 5 μm）；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱；流速为0.8 ml/min；检测波长为300 nm；柱温为35℃。结果 通过中药色谱指纹图谱相似度评价系统（2004A），确定了清脂胃舒佐餐水溶性成分的HPLC指纹图谱共有模式，标定了26个共有峰，整体相识度为0.934~0.995；并测定了10批制剂中绿原酸、柚皮苷、橙皮苷的含量分别为0.672、0.194、1.247和0.532 mg/g，RSD分别为1.35%、1.89%、0.69%和0.79%。结论 清脂胃舒佐餐水溶性成分的HPLC指纹图谱及其中没食子酸、绿原酸、柚皮苷、橙皮苷的含量检测方法简便、稳定、科学，为本品的质量控制提供了科学依据。 |
| 中文关键词:清脂胃舒佐餐茶 高效液相色谱 指纹图谱 没食子酸 绿原酸 柚皮苷 橙皮苷 |
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| Study on fingerprint of Qingzhiweizuocan Tea and assay of four components by HPLC method |
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| Abstract:Objective To establish HPLC fingerprint for water soluble components and assay gallic acid, chlorogenic acid, naringin, aurantiamarin in Qingzhiweizuocan Tea, Methods High Performance Liquid Chromatography(HPLC) analysis was performed on Kromasil C18 column (250 mm×4.6 mm, 5 μm) with a mixture of acetonitrile and 0.1% phosphoric acid solution as mobile phase in gradient elution. The flow rate was 0.8 ml/min, the detection wavelength at 300 nm and the column temperature at 35℃. Results Based on the chromatographic fingerprint similarity evaluation system (2004A) analysis, the characteristic fingerprint peak of the water-soluble components in Qingzhiweizuocan Tea was composed of 26 chromatographic peaks. The overall similarity degree is from 0.934 to 0.995. Ten batches of Qingzhiweizuocan Tea were assayed. The average content of gallic acid, chlorogenic acid, naringin, aurantiamarin were 0.672, 0.194, 1.247, 0.532 mg/g respectively. The RSDs (%) were 1.35, 1.89, 0.69, 0.79. Conclusion This method is simple, accurate and with good repeatability. This study provided a scientific foundation for quality evaluation and control of Qingzhiweizuocan Tea. |
| keywords:Qingzhiweizuocan Tea HPLC fingerprint gallic acid chlorogenic acid naringin aurantiamarin |
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