| 胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究 |
| 投稿时间：2017-12-01 修订日期：2018-01-04 点此下载全文 |
| 引用本文：黄瑾,刘景雪,曾颖,袁芳.胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究[J].药学实践杂志,2018,36(2):126~130 |
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| 基金项目:上海市卫生和计划生育委员会科研课题资助项目（201440461），浦东新区卫生系统学科带头人培养计划（PWRd2014-11） |
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| 中文摘要:目的 研究TRPV1受体的表达变化对表皮生长因子受体（EGFR）蛋白表达的调控作用，及其对相关细胞功能的影响。方法 利用过表达技术和siRNA技术研究TRPV1表达变化对EGFR蛋白表达的影响，用CCK-8实验检测细胞的增殖，并用Real-time PCR实验对功能相关的癌基因Akt2和K-ras的表达进行检测。结果 在胰腺癌细胞中TRPV1过表达或是受激动剂激活后，EGFR的蛋白含量下调；反之，干扰TRPV1表达会使EGFR的蛋白表达增加；过表达TRPV1的胰腺癌细胞其增殖速率减缓；过表达TRPV1的胰腺癌细胞中原癌基因Akt2和K-ras的表达明显受到抑制，反之则激活其表达。结论 胰腺癌细胞中TRPV1的表达调控了EGFR的蛋白含量，并对与其功能密切相关的细胞增殖和癌基因表达也有调节作用。 |
| 中文关键词:胰腺癌细胞 TRPV1 表皮生长因子受体 细胞增殖 |
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| Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells |
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| Abstract:Objective To evaluate the regulation of the TRPV1 receptor on the expression of EGFR protein and the effects on related cell functions. Methods Human pancreatic cancer cells PANC-1 were cultured and treated by over-expression and siRNA. Cell proliferations were detected by CCK-8 experiment. RT-PCR test was used to detect the expression of oncogenes Akt2 and K-ras. Results EGFR protein was down-regulated by TRPV1 over-expression or by agonist activation in pancreatic cancer cells. EGFR protein was increased by the interference of TRPV1 expression. The proliferation rate of pancreatic cancer cells was decreased and Akt2, K-ras were significantly inhibited by TRPV1 over-expressing. Conclusion The expression of TRPV1 in pancreatic cancer cells regulated the EGFR protein content. The cell proliferation and oncogene expression were inhibited by TRPV1 over-expressing. |
| keywords:transient receptor potential vanilloid 1 EGFR pancreatic cancer cells cell proliferation |
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