| HPLC法测定扶正平消胶囊中芍药苷的含量 |
| 投稿时间：2017-04-10 修订日期：2017-12-29 点此下载全文 |
| 引用本文：付振贺,赵亮,周瑾,王新霞,张国庆,黄玉凤.HPLC法测定扶正平消胶囊中芍药苷的含量[J].药学实践杂志,2018,36(1):68~70,79 |
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| 基金项目:军队医疗机构制剂单品种研究：扶正平消胶囊标准研究（14TG0700） |
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| 中文摘要:目的 建立扶正平消胶囊中芍药苷含量的测定标准。方法 采用HPLC法进行含量测定，色谱柱为Agilent Zorbax SB-C18柱（150 mm×4.6 mm，5 μm），流动相：乙腈-5 mmol/L磷酸二氢钠水溶液（10：90），流速：1.0 ml/min，柱温：25℃；检测波长：230 nm，进样量10 μl，运行时间25 min。结果 芍药苷在25.0~500.0 μg/ml范围内线性关系良好（r=0.999 9），线性方程：Y=12.65X+43.54，平均加样回收率为92.69%，RSD为1.77%。结论 该方法操作易行、结果可靠、重复性好，可用于扶正平消胶囊中芍药苷的含量测定。 |
| 中文关键词:高效液相色谱 扶正平消胶囊 芍药苷 含量测定 |
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| Determination of paeoniflorin in Chinese traditional herbal preparation Fuzhengpingxiao capsule using HPLC method |
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| Abstract:Objective To determine the content of paeoniflorin in Fuzhengpingxiao capsule by HPLC method. Methods The content of paeoniflorin were determined by HPLC, and the column was an Agilent Zorbax SB-C18 column (150 mm×4.6 mm, 5 μm); The mobile phase was acetonitrile and 5 mmol/L sodium dihydrogen phosphate (10:90), and the flow rate was set at 1 ml/min. Column temperature was 25℃, detection wavelength was 230 nm, injection volume was 10 μl, and running time was 25 min. Results Paeoniflorin was linear (r=0.999 9) in the concentration of 25.0-500.0 μg/ml, the linear equation was Y=12.65X +43.54, and the average recovery was 92.69% with RSD value was 1.77%. Conclusion The method was easy to operate, reliable and reproducible, which could be used in the determination of paeoniflorin in FuzhengPingxiao capsule. |
| keywords:HPLC FuzhengPingxiao capsule paeoniflorin determination |
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