| 槲皮素与锌离子结合性质及抗氧化活性研究 |
| 投稿时间：2016-12-08 修订日期：2017-04-25 点此下载全文 |
| 引用本文：邱丽娟,孟欣,候升书,杨根金,仲维清.槲皮素与锌离子结合性质及抗氧化活性研究[J].药学实践杂志,2018,36(1):50~54 |
| 摘要点击次数: 2092 |
| 全文下载次数: 2338 |
|
| 基金项目:国家自然科学基金（21273283） |
|
| 中文摘要:目的 研究槲皮素与其锌离子（Zn2+）配合物的抗氧化性能。方法 通过紫外/可见分光光度法测定槲皮素与Zn2+的结合比例及结合常数，结合核磁共振技术确定其结合位点，以1，1-二苯基苯肼（DPPH）为探针，比较研究槲皮素与其Zn2+配合物的抗氧化性能。结果 当槲皮素中低于2个酚羟基被中和时，槲皮素与Zn2+的饱和结合比为2：1；当超过3个酚羟基被中和时，槲皮素与Zn2+的结合比为1：1，此时槲皮素-Zn2+的表观结合常数为2.42×106 M-1。槲皮素的3'-O、4'-O参与了与Zn2+的配位。槲皮素-Zn2+配合物清除DPPH自由基的活性比槲皮素本身高出2.3倍。结论 Zn2+可与槲皮素形成稳定的配合物，且其比槲皮素本身具有更高效的抗氧化性能，这为传统中药槲皮素的应用提供了新思路。 |
| 中文关键词:槲皮素 锌离子 结合常数 抗氧化 |
| |
| Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant activities |
|
|
| Abstract:Objective To study the anti-oxidation properties of Zn2+-quercetin complexes. Methods The binding stoichiometric ratios and the binding constant of Zn2+ to quercetin were measured with UV-Vis spectroscopy. The binding sites on quercetin were determined via 1H NMR spectroscopy. The antioxidant activities of Zn2+-quercetin complexes were compared to quercetin by scavenging DPPH radical method. The binding ratio of Zn2+ to quercetin depends on neutralization of phenol groups of quercetin. Results The binding ratio of Zn2+ to quercetin is 1:2 when less than two OH groups were deprotonated. The binding ratio is 1:1 when more than three OH groups were deprotonated. The apparent binding constant is 2.42×106 M-1 for the 1:1 Zn2+-quercetin complex. The 3'-OH and 4'-OH of quercetin are involved in the Zn2+ binding. The scavenging DPPH radical activity of Zn2+-quercetin complex is 2.3 times of quercetin. Conclusion These results provide a new insight to expand applications of this traditional Chinese medicine. |
| keywords:quercetin Zn2+ ion binding constant antioxidant activity |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
