| HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚的含量 |
| 投稿时间：2017-05-15 修订日期：2017-07-31 点此下载全文 |
| 引用本文：王娅俐,彭贤东,郝伟迤,王丹,郭廷东,唐志立.HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚的含量[J].药学实践杂志,2017,35(6):551~553,576 |
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| 中文摘要:目的 建立以HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚含量的方法。方法 色谱柱为SHIADZU-GL Inertsil® ODS-3 RP C18，流动相为甲醇-水（60：40），检测波长为285 nm，流速为1.0 ml/min，柱温为40℃，外标法定量。结果 醋酸地塞米松、樟脑和苯酚的线性范围分别为4.024~40.24、101.7~2 033和10.38~425.2 μg/ml，相关系数r >0.999 5；三者的平均回收率分别为101.2%（RSD为0.56%）、99.89%（RSD为0.72%）、100.2%（RSD为0.97%）；重复性试验中RSD均小于1.5%。结论 本方法简便、准确，重复性好，可用于地米樟酚乳膏的质量控制。 |
| 中文关键词:高效液相色谱 乳膏 醋酸地塞米松 樟脑 苯酚 含量测定 |
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| Simultaneous determination of dexamethasone acetate, camphor and phenol in compound cream by HPLC method |
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| Abstract:Objective To establish a HPLC method for simultaneously determining the content of dexamethasone acetate, camphor and phenol in compound cream.Methods The separation was performed on a SHIADZU-GL Inertsil® ODS-3 RP C18 analytical column with the mobile phase consisting of methanol-water (60:40). The flow rate was 1.0 ml/min. The detection wave length was 285 nm and the column temperature was 40℃.Results Dexamethasone acetate,camphor and phenol showed good linearity (r>0.999 5, n=7) within the concentration range of 4.024-40.24,101.7-2 033 and 10.38-425.2 μg/ml,respectively. The average recovery of dexamethasone acetate, camphor and phenol was 101.2%(RSD was 0.56%),99.89%(RSD was 0.72%),100.2%(RSD was 0.97%),respectively. Moreover, the RSDs were less than 1.5% in the repeated tests. Conclusion The method was simple, quick and accurate. It is suitable for the quality control of dexamethasone acetate camphor and phenol cream. |
| keywords:HPLC cream dexamethasone acetate camphor phenol determination |
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