| HPLC法测定仙灵骨葆胶囊中5种成分的含量 |
| 投稿时间：2016-06-28 修订日期：2016-11-01 点此下载全文 |
| 引用本文：陈宗良,吕晓霞,陈桂茜,陈捷.HPLC法测定仙灵骨葆胶囊中5种成分的含量[J].药学实践杂志,2017,35(5):444~446,452 |
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| 基金项目:金华市科技局科技项目资助（2015-3-031） |
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| 中文摘要:目的 采用多波长HPLC法建立仙灵骨葆胶囊中淫羊藿苷、朝藿定C、川续断皂苷Ⅵ、补骨脂素及异补骨脂素的含量测定方法。方法 采用月旭Ultimate® XB-C18柱，流动相采用乙腈-水系统，梯度洗脱；柱温：30 ℃；检测波长：淫羊藿苷、朝藿定C、川续断皂苷Ⅵ为212 nm，补骨脂素、异补骨脂素为246 nm。结果 5种成分均能达到基线分离，各成分的进样量分别在：淫羊藿苷0.008 2~0.328 μg（r=0.999 5）、朝藿定C 0.055 6~2.224 μg（r=0.999 6）、川续断皂苷Ⅵ 0.144 1~5.764 μg（r=0.999 6）、补骨脂素0.005 4~0.215 2 μg（r=0.998 0）、异补骨脂素0.006 6~0.265 6 μg（r=0.998 5）范围内线性关系良好，平均回收率分别为97.59%、98.58%、98.11%、97.86%、98.22%，RSD均小于2.0%。结论 该方法操作简便、分离良好、数据准确、灵敏度高，可用于仙灵骨葆胶囊的多指标成分定量测定。 |
| 中文关键词:高效液相色谱 仙灵骨葆胶囊 淫羊藿苷 朝藿定C 川续断皂苷Ⅵ 补骨脂素 异补骨脂素 |
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| One step assay for five components in Xianlinggubao capsule by HPLC method |
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| Abstract:Objective To assay Icariine, Epimedin C, asperosaponin Ⅵ, psoralen and angelicin in Xianlinggubao capsules via multi-wavelength HPLC method. Methods Separation was carried out on Welch Ultimate® XB-C18 column. The mobile phase was acetonitrile-water system and a linear gradient elution was used. The column temperature was 30 ℃. The detection wavelength for Icariine, Epimedin C,asperosaponin Ⅵ was set at 212 nm, psoralen and angelicin at 246 nm. Results Five components reached baseline separation, the linearity was good when sample size was in the range of 0.008 2-0.328 μg for Icariine(r=0.999 5), 0.055 6-2.224 μg for Epimedin C (r=0.999 6), 0.144 1-5.764 μg for asperosaponin Ⅵ(r=0.999 6), 0.005 4-0.215 2 μg for psoralen(r=0.998 0), 0.006 6-0.265 6 μg for angelicin(r=0.998 5). The average recoveries were 97.59%, 98.58%, 98.11%, 97.86%, 98.22% respectively. The RSDs of recovery were all less than 2.0%. Conclusion This method is simple, accurate, with good separation, high sensitivity for the assay of multiple components in Xianlinggubao capsule. |
| keywords:HPLC Xianlinggubao capsule Icariine Epimedin C asperosaponin Ⅵ psoralen angelicin |
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