| HPLC法测定积雪草配方颗粒中羟基积雪草苷和积雪草苷含量 |
| 投稿时间：2016-12-21 修订日期：2017-03-10 点此下载全文 |
| 引用本文：杨梅,李峻媛,吴家彬,玉秋萍,韦敏灵,韦红言.HPLC法测定积雪草配方颗粒中羟基积雪草苷和积雪草苷含量[J].药学实践杂志,2017,35(4):359~361 |
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| 基金项目:2014年自治区重大科技专项（桂科攻14124002-13）；南宁市本级科学研究与技术开发计划项目（20153326）；南宁市人才小高地项目（2015023） |
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| 中文摘要:目的 建立积雪草配方颗粒中羟基积雪草苷和积雪草苷的HPLC含量测定方法。方法 色谱柱为UltimateAQ-C18 柱（4.6 mm×250 mm，5 μm），以乙腈（A）-2 mmol/Lβ-环糊精溶液为流动相进行梯度洗脱（0~30 min： 21% A→23% A；30~60 min：23% A→25% A），流速1.0 ml/min，柱温30 ℃，检测波长205 nm。结果 羟基积雪草苷和积雪草苷分别在0.187 7~3.754 μg和0.184 3~3.686 μg范围内与峰面积呈良好的线性关系（r>0.999 5）。经专属性、重复性、精密度、加样回收率、稳定性实验，结果均符合《中华人民共和国药典（2015年版）》方法验证的要求。结论 该含量测定方法适用于积雪草配方颗粒中羟基积雪草苷和积雪草苷的含量检测。 |
| 中文关键词:积雪草 配方颗粒 含量测定 羟基积雪草苷 积雪草苷 高效液相色谱 |
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| Determination of madecassoside and asiaticoside in Centella asiatica formula granules by HPLC method |
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| Abstract:Objective To establish a HPLC method for determination of madecassoside and asiaticoside in Centella asiatica formula granules. Methods Chromatographic separation was performed on Ultimate AQ-C18 column(4.6 mm×250 mm,5 μm) with mobile phase of acetonitrile(A)-2 mmol/L β-cyclodextrin(0~30 min:21% A→23% A; 30~60 min:23% A→25% A).The flow rate was set at 1.0 ml/min, the column temperature at 30 ℃ and detection wavelength at 205 nm. Results Madecassoside and asiaticoside showed good linearity (r>0.9995) in the ranges of 0.187 7~3.754 μg and 0.184 3~3.686 μg respectively. The specificity, repeatability, precision,recovery and stability were satisfied to the method validation requirements of China Pharmacopoeia. Conclusion The method can determine madecassoside and asiaticoside in Centella asiatica formula granules. |
| keywords:Centella asiatica formula granules content determination madecassoside asiaticoside HPLC |
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