| 益视明目颗粒质量标准研究 |
| 投稿时间：2016-10-16 修订日期：2017-01-06 点此下载全文 |
| 引用本文：缪婧,童晓东.益视明目颗粒质量标准研究[J].药学实践杂志,2017,35(4):355~358 |
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| 中文摘要:目的 建立益视明目颗粒的质量标准。方法 用薄层色谱法（TLC）鉴别制剂中的枸杞子、丹参；用高效液相色谱法（HPLC）定量测定丹参素钠的含量。色谱柱：Kromasil C18柱（4.6 mm×150 mm，5 μm）；流速：0.4 ml/min；检测波长：280 nm；柱温：30 ℃；进样量：10 μl；流动相：甲醇-0.5%醋酸水溶液（10∶90）。结果 薄层色谱斑点清晰可见、阴性干扰小，可用于鉴别益视明目颗粒中的枸杞、丹参；丹参素钠在2.00~60.00 μg/ml范围内线性关系良好，r=0.999 7（n=6），平均加样回收率105.6%，RSD=1.60%。结论 建立的方法简便、准确、可靠性高、重现性好，可作为控制益视明目颗粒质量的有效方法。 |
| 中文关键词:益视明目颗粒 质量标准 丹参素钠 |
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| The quality standard research for YI SHI MINGMU granules by HPLC method |
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| Abstract:Objective To establish the quality standard for YI SHI MINGMU granules. Methods TLC method was used to identify Lycium barbarum L and Salvia miltiorrhiza. HPLC method was used to quantitatively analyze the concentration of Tanshinol sodium. The analysis was carried out on a column of Kromasil C18(4.6 mm×150 mm,5 μm)with a mobile phase of methanol and 0.5 % acetic acid at the flow rate of 0.4 ml/min. Column temperature was 30 ℃. The detection wavelength was 280 nm. Results The linearity range of Tanshinol sodium was 2.00~60.00 μg/ml,r2=0.999 7(n=6),with the average recoveries of 105.62%, RSD=1.60%. Conclusion This method is accurate, simple, reliable and reproducible. It can be used for the quality control of YI SHI MINGMU granules. |
| keywords:YI SHI MINGMU granules quality standard Tanshinol sodium |
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