| 高效液相色谱法测定降脂护肝胶囊中葛根素的含量 |
| 投稿时间：2015-03-13 修订日期：2015-07-12 点此下载全文 |
| 引用本文：郑巍,倪东杰,韦庆,向会,胡兰兰,孙亮.高效液相色谱法测定降脂护肝胶囊中葛根素的含量[J].药学实践杂志,2017,35(3):259~261 |
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| 中文摘要:目的 建立高效液相色谱法测定降脂护肝胶囊中葛根素的含量。方法 色谱柱为Waters Symmetry C18柱（4.6 mm×250 mm，5 μm），流动相为乙腈-1%甲酸（11：89），检测波长250 nm，流速1.0 ml/min，柱温30 ℃，进样量10 μl。结果 葛根素在2~40 μg/ml范围内（r=0.999 8）呈良好的线性关系，精密度实验中RSD均小于2%，加样回收率在98%~103%。结论 该测定方法简便、准确，分离效果好，可用于降脂护肝胶囊的质量控制。 |
| 中文关键词:高效液相色谱法 降脂护肝胶囊 葛根素 含量测定 |
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| Determination of pueratin in Jiangzhi Hugan capsule by HPLC |
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| Abstract:Objective To develop a HPLC method for determination of pueratin. Methods The separation was carried out on a Waters Symmetry C18 column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 1% formic acid(11:89), the detection wavelength was set at 250 nm, the flow rate was 1.0 ml/min, the column temperature was 30 ℃ and the injection volume was 10 μl. Results The linearity was obtained over 2~40 μg/ml (r=0.999 8) for pueratin. The RSD of precision were less than 2%. The average recovery was between 98% and 103%. Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Jiangzhi Hugan capsule. |
| keywords:HPLC Jiangzhi Hugan capsule pueratin content determination |
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