| 蒲地灌肠液中总多糖含量测定与方法学验证 |
| 投稿时间：2016-02-17 修订日期：2016-07-19 点此下载全文 |
| 引用本文：王艺红,陈锦珊,许彩燕,郑绍忠.蒲地灌肠液中总多糖含量测定与方法学验证[J].药学实践杂志,2017,35(3):248~251 |
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| 中文摘要:目的 建立蒲地灌肠液中总多糖的含量测定方法。方法 采用苯酚-硫酸法显色，紫外分光光度法在488.8 nm波长处测定总多糖的含量。结果 总多糖在0~22.635 mg/L范围内呈良好线性关系；其回归方程为Y=0.062 06 X-0.003 34（r=0.999 8），回收率为98.36%，RSD为2.34%（n=6）。结论 该方法灵敏、快速、准确、可靠，可用于蒲地灌肠液中总多糖含量的测定。 |
| 中文关键词:蒲地灌肠液 总多糖 苯酚-硫酸法 紫外分光光度法 含量测定 |
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| The methodology validation and content assay of total polysaccharides in Pudi Enema |
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| Abstract:Objective To establish the assay method for the total polysaccharide in Pudi Enema. Methods Phenol-sulfuric acid method was used for chromogenic reaction. The content of total polysaccharide was measured by UV spectrophotometry at 488.8 nm. Results The total polysaccharides calibration curve was at the range of 0~22.635 mg/L, with regression function being Y=0.062 06 X-0.003 34(r=0.999 8). The recovery of calycosin was 98.36%(RSD=2.34%). Conclusion This method is sensitive,rapid,accurate and reliable. It can be used to assay the content of total polysaccharide in Pudi Enema. |
| keywords:Pudi Enema phenol-sulfuric acid method total polysaccharides UV spectrophotometry content assay |
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