| 依托泊苷壳聚糖胶束的制备及壳聚糖促进依托泊苷肠吸收作用的研究 |
| 投稿时间：2016-05-15 修订日期：2016-11-13 点此下载全文 |
| 引用本文：陈松,王彧杰,王欢,原永芳.依托泊苷壳聚糖胶束的制备及壳聚糖促进依托泊苷肠吸收作用的研究[J].药学实践杂志,2017,35(3):243~247 |
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| 中文摘要:目的 制备依托泊苷壳聚糖胶束，并研究壳聚糖对依托泊苷肠吸收的促进作用。方法 用透析法制备依托泊苷壳聚糖胶束，建立依托泊苷HPLC含量测定方法，测定了其包封率与载药量；采用大鼠在体肠循环实验，研究不同剂量壳聚糖对依托泊苷全肠段和各个肠段吸收的影响。结果 壳聚糖胶束平均粒径为139.5 nm，多分散系数为0.569；依托泊苷标准曲线为A=8 436.8C-4 963.8，r=1.000 0，日内、日间精密度符合要求；包封率为（47.3±2.84）%，载药量为（1.10±1.27）%；随着壳聚糖浓度的增加，依托泊苷在全肠段的单位面积吸收量有不同程度的增加；壳聚糖对依托泊苷的吸收促进作用存在着肠道特异性，作用大小顺序：回肠 >空肠 >十二指肠。结论 在十二指肠、空肠和回肠，壳聚糖都不同程度促进了药物的吸收，且在空肠和回肠有显著性的影响。 |
| 中文关键词:壳聚糖 依托泊苷 肠吸收 |
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| Preparation of the etoposide chitosan micelle and the effect of chitosan on etoposide intestinal absorption |
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| Abstract:Objective To prepare the etoposide chitosan micelle, and investigate the effect of chitosan on etoposide intestinal absorption. Methods The etoposide chitosan micelle was prepared by dialysis. The drug encapsulation efficiency and drug loading efficiency were determined by HPLC. The intestine in rats was cannulated for in situ recirculation. The effects of different chitosan doses on the intestinal drug absorption and the effects of chitosan on the drug absorption at different intestinal locations were studied. Results The average particle size of etoposide chitosan micelle was 139.5 nm. The multi-dispersion coefficient was 0.569. The standard curve of etoposide was A = 8 436.8 C-4 963.8,r=1.000 0. The intra-and inter-day precision values meetthe requirement. The drug encapsulation efficiency was (47.3±2.84)% and drug loading efficiency was (1.10±1.27)%. With the increase of the chitosan concentration, the absorption capacity of the unit area in the whole intestine was increased in different degrees. Chitosan exhibits its effects on etoposide absorptionat different intestinal sections in the following order: ileum >jejunum >duodenum. Conclusion Chitosan promoted etoposide absorption induodenum, jejunum and ileum, especially in jejunum and ileum. |
| keywords:chitosan etoposide intestinal absorption |
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