| 催化菘蓝活性木脂素生物合成的漆酶基因家族生物信息学分析 |
| 投稿时间：2017-02-21 修订日期：2017-04-15 点此下载全文 |
| 引用本文：陈亮,李卿,陈军峰,冯婧娴,张磊,陈万生.催化菘蓝活性木脂素生物合成的漆酶基因家族生物信息学分析[J].药学实践杂志,2017,35(3):208~214 |
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| 基金项目:国家自然科学基金资助项目（81325024，81303160，81673550，31670292） |
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| 中文摘要:目的 从转录组数据库中发掘并分析了参与菘蓝落叶松脂素生物合成的关键合成酶之一——漆酶（laccase）的基因序列，并进行生物信息学分析，为以后的功能研究提供可靠依据。方法 利用生物信息学分析软件，从已有菘蓝转录组数据库中寻找漆酶并分析其各项特征，主要包括理化性质、同源性分析、蛋白结构分析和茉莉酸甲酯（MeJA）诱导后表达特征等分析。结果 菘蓝漆酶基因（Iilacs）中的Iilac3和Iilac5在MeJA诱导下的转录水平与药效成分的积累相一致，推测其可能为参与落叶松脂素合成的潜在的功能基因。结论 通过对菘蓝漆酶进行详细的生物信息学分析，可为今后功能蛋白的筛选和进一步研究其生理生化机制和结构特征打下坚实的基础。 |
| 中文关键词:菘蓝 漆酶 生物信息学 落叶松脂素 |
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| Bioinformatics analysis of laccases involved in active lignans' biosynthesis in Isatis indigotica |
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| Abstract:Objective Identifying laccases, as one of the key synthetases in the lariciresinol biosynthetic pathway, by analyzing the transcriptome sequencing results in Isatis indigotica would provide a dependable foundation for later functional study of Isatis indigotica's laccases. Methods Bioinformatical softwares and kinds of analytical methods online were used to find out the characteristics of the laccases from I. indigotica, including physical and chemical properties, homology, and the properties after induction of MeJA. Results The transcriptional results showed that Iilac3 and Iilac5 from I. indigotica were corresponded with the accumulation of the effective metabolites, making them the potential functional genes participated in lariciresinol synthesis. Conclusion Through the detailed bioinformatical analysis of Iilacs,which laid a solid foundation for the further study of the physiological and biochemical mechanisms and structural characteristics of the functional proteins. |
| keywords:Isatis indigotica Fort laccase bioinformatics lariciresinol |
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