| HPLC法和UV法测定复方枣参片中斯皮诺素和总皂苷含量 |
| 投稿时间：2016-12-22 修订日期：2017-02-28 点此下载全文 |
| 引用本文：郑峰,阙晓,苏靖,丁丽燕,聂曲,邱明丰.HPLC法和UV法测定复方枣参片中斯皮诺素和总皂苷含量[J].药学实践杂志,2017,35(2):134~137,173 |
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| 中文摘要:目的 建立复方枣参片中斯皮诺素和总皂苷的含量测定方法。方法 HPLC法测定斯皮诺素采用Agilent Zorbax SB C18柱（250 mm×4.6 mm， 5μm），柱温25℃，流动相为乙腈-水，采用梯度洗脱，流速为1.0 ml/min，检测波长为335 nm。UV法测定总皂苷采用Amberlite XAD-2大孔树脂柱层析后，香草醛-冰醋酸法显色测定，检测波长为560 nm。结果 斯皮诺素和总皂苷的线性范围分别为20~100μg/ml（r=0.9990）和40~200μg/ml（r=1.0000），准确度平均值分别为99.55%和99.85%。结论 本方法准确可靠，可作为复方枣参片的质量控制依据。 |
| 中文关键词:复方枣参片 高效液相色谱法 紫外-可见光分光光度法 斯皮诺素 总皂苷 |
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| Determination of spinosin and total saponins in compound Zaoshen tablet by HPLC and UV |
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| Abstract:Objective To establish methods for the determination of spinosin and total saponins in compound Zaoshen tablet.Methods The separation of spinosin was performed on Agilent Zorbax SB C18 column (250 mm×4.6 mm, 5 μm) at 30℃ with water and acetonitrile as mobile phase for gradient elution at the flow rate of 1.0 ml/min. The detection wavelength was 335 nm. Determination of total saponins was fulfilled by column chromatography followed by UV spectrometer with vanillin glacial acetic acid colorimetry. The detection wavelength was 560 nm.Results Linear range of spinosin was 20-100 μg/ml (r=0.999 0) and total saponins was 40-200 μg/ml (r=1.000 0). The average recoveries for accuracy tests were 99.55% and 99.85% respectively.Conclusion Those methods are accurate and reliable. They can be used for the quality control of compound Zaoshen tablet. |
| keywords:compound Zaoshen tablet HPLC UV spinosin total saponins |
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