| 柱前衍生RP唱HPLC法同时测定珐菲亚中14种水解氨基酸 |
| 投稿时间：2016-10-13 修订日期：2016-12-16 点此下载全文 |
| 引用本文：陈哲,路娟,彭纪铭,柴瑞平,陈曦.柱前衍生RP唱HPLC法同时测定珐菲亚中14种水解氨基酸[J].药学实践杂志,2017,35(2):130~133 |
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| 基金项目:国家自然科学基金面上项目（81673667）；国家重大科技专项（2014ZX09201022-006） |
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| 中文摘要:目的 建立柱前衍生反相高效液相色谱法同时测定珐菲亚中14种水解氨基酸含量的方法。方法 采用Waters XBridge Shield RP18色谱柱（4.6 mm×250 mm，5μm），异硫氰酸苯酯为柱前衍生化试剂，梯度洗脱，流速0.8 ml/min，检测波长254 nm，柱温25℃。结果 14种氨基酸在一定浓度范围内呈良好的线性关系，相关系数r值均>0.9990，加样回收率（n=6）为90.2%~105.1%，氨基酸衍生溶液在24 h内稳定。结论 本实验建立的方法可靠，可用于珐菲亚中氨基酸的含量测定。 |
| 中文关键词:珐菲亚 柱前衍生 反相高效液相色谱法 氨基酸 异硫氰酸苯酯 |
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| Simultaneous determination of 14 amino acids in Pfaffia by RP-HPLC with precolumn derivatization |
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| Abstract:Objective To develop a HPLC method for determining 14 hydrolyzed amino acids in Pfaffia.Methods The sample was derivatized with phenyl isothiocyanate (PITC). Amino acids were separated on Waters XBridge Shield RP18 (4.6 mm×250 mm, 5 μm) column at the flow rate of 0.8 ml/min, detected at 254 nm. The column temperature was 25℃.Results The response was linear for 14 amino acids with a correlation coefficient r>0.999 0. The average recoveries (n=6) were 90.2%-105.1%. Amino acids derivative solution remained stable in 24 hours.Conclusion This well-established method is very reliable. It can be used as a quantitative determination method for 14 amino acids in Pfaffia. |
| keywords:Pfaffia pre-column derivatization RP-HPLC amino acids phenyl isothiocyanate |
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