| 新疆白刺叶中异鼠李素-3-芸香糖苷的含量分析 |
| 投稿时间：2016-01-02 修订日期：2016-06-23 点此下载全文 |
| 引用本文：蒋益萍,张巧艳,冯缨,王恒,王琪,辛海量.新疆白刺叶中异鼠李素-3-芸香糖苷的含量分析[J].药学实践杂志,2016,34(6):522~525 |
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| 基金项目:国家自然科学基金联合基金项目（U1203104） |
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| 中文摘要:目的 分析新疆产白刺叶中黄酮类成分异鼠李素-3-芸香糖苷的含量，对新疆白刺的品质评价和质量控制提供依据。方法 采用HPLC法对新疆白刺叶中异鼠李素-3-芸香糖苷进行含量分析。色谱柱：Diamonsil C18柱（4.6 mm×250 mm，5 μm）；流速：1 ml/min；柱温：26℃；检测波长：360 nm。结果 新疆白刺叶中异鼠李素-3-芸香糖苷在0.007 813~1 mg/ml的范围内线性关系良好，平均加样回收率为100.13%，RSD=3.02%。结论 异鼠李素-3-芸香糖苷在白刺叶中的含量存在较大的种内和种间差异。该方法操作简单，检测结果准确，重复性好，可作为新疆白刺叶中异鼠李素-3-芸香糖苷的定量分析方法。 |
| 中文关键词:白刺 黄酮 异鼠李素-3-芸香糖苷 高效液相色谱 |
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| Quantitative analysis of isorhamnetin-3-rutinoside in leaves of Nitraria Spp. from Xinjiang region |
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| Abstract:Objective To provide supporting data for the quality control and evaluation of Nitraria Spp. from Xinjiang region by analyzing the content of isorhamnetin-3-rutinoside in its leaves. Methods HPLC method was used for the determination of isorhamnetin-3-rutinoside in leaves of Nitraria Spp. Results There is a good linear relationship in the range of 0.007813~1 mg/ml. The average recovery rate is 100.13% (RSD=3.02%). Conclusion There were large interspecific and intraspecific differences of isorhamnetin-3-rutinoside content in leaves of Nitraria Spp from Xinjiang region. A simple method was identified to give accurate and reproducible results. This method can be used for quantitative analysis of isorhamnetin-3-rutinoside content in leaves of Nitraria Spp. |
| keywords:Nitraria Spp. flavone isorhamnetin-3-rutinoside HPLC |
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