| 高效液相色谱法测定乐肤口服液中苦参碱的含量 |
| 投稿时间：2015-02-04 修订日期：2015-10-20 点此下载全文 |
| 引用本文：吴相雷,岳明,黄皓,陆文铨,朴淑娟,张凤.高效液相色谱法测定乐肤口服液中苦参碱的含量[J].药学实践杂志,2016,34(5):443~446 |
| 摘要点击次数: 1538 |
| 全文下载次数: 1185 |
|
| 基金项目:中药口服液类特色制剂的质量标准研究（14ZJZ02-4） |
|
| 中文摘要:目的 建立HPLC法测定乐肤口服液中苦参碱含量的方法。方法 色谱柱为Diamonsil Platisil NH2柱（4.6 mm×250 mm， 5 μm），流动相为乙腈-异丙醇-3%磷酸水溶液（84：4：12）；流速为1.2 ml/min；进样量5 μl；检测波长205 nm。结果 苦参碱在54.50~872.00 μg/ml浓度范围内线性关系良好，r=0.999 1；平均回收率为99.82%， RSD=1.12%。结论 该法简便、稳定可靠、重复性好，为控制乐肤口服液的质量标准提供了可借鉴的依据。 |
| 中文关键词:高效液相色谱法 苦参碱 乐肤口服液 |
| |
| Determination of matrine in Lefu oral liquid by HPLC |
|
|
| Abstract:Objective To establish a high performance liquid chromatography method for the determination of matrine in Lefu oral liquid. Methods The HPLC method was performed on a Diamonsil Platisil NH2 column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-isopropyl alcohol-3% phosphoric acid solution (84:4:12). The flow rate was 1.2 ml/min. The sample injection volume was 5 μl. The detective wavelength was 205 nm. Results The calibration curve of matrine showed good linear response ranged from 54.50 to 872.00 μg/ml with r=0.999 1. The average recovery of spiked samples for matrine was 99.82% while the relative standard deviation for repetitions was 1.12%. Conclusion The method was simple, reliable and repeatable, which could be used for the quantitative determination of matrine of Lefu oral liquid. |
| keywords:HPLC matrine Lefu oral liquid |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
