| 高效液相色谱法测定壮骨颗粒中补骨脂素与异补骨脂素的含量 |
| 投稿时间：2015-12-20 修订日期：2016-07-07 点此下载全文 |
| 引用本文：蒲瑾,徐良燕,陈若华.高效液相色谱法测定壮骨颗粒中补骨脂素与异补骨脂素的含量[J].药学实践杂志,2016,34(5):441~442 |
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| 中文摘要:目的 建立高效液相色谱法测定壮骨颗粒中补骨脂素和异补骨脂素的含量。方法 色谱柱为Agilent Zorbax C18柱（4.6 mm×250 mm，5 μm），流动相为甲醇-水（45：55），检测波长为245 nm，流速为1.0 ml/min，柱温为25 ℃，进样量为10 μl。采用70%乙醇水浴方法提取壮骨颗粒中的补骨脂素和异补骨脂素。结果 补骨脂素与异补骨脂素在3.75~40 μg/ml范围内呈良好的线性关系，对方法精密度（n=6）、重复性（n=6）和稳定性（12 h）进行考察，相对标准偏差（RSD）均小于2%。加样回收率（n=6）为94%~105%。结论 该测定方法简便、快速、准确，可用于壮骨颗粒的临床快速质量控制。 |
| 中文关键词:高效液相色谱法 壮骨颗粒 补骨脂素 异补骨脂素 |
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| HPLC method to assay the content of psoralen and angelicin in Zhuanggu granules |
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| Abstract:Objective To develop a HPLC method for psoralenand angelicin content assay in Zhuanggu granules. Methods Agilent Zorbax C18 column(4.6 mm×250 mm, 5 μm) was used with methanol and water(45:55) as mobile phase. The detection wavelength was set at 245 nm. The flow rate was 1.0 ml/min and column temperature at 25 ℃. The injection volume was 10 μl.Psoralenand angelicin were extracted with 70% ethanol under water bath. Results The linearity was obtained over 3.75~40 μg/mlfor psoralenand angelicin. The RSDs were less than 2%. The average recovery was between 94% and 105%. Conclusion This simple and accurate HPLC method was suitable for the quality control of Zhuanggu granules. |
| keywords:HPLC Zhuanggu granules psoralen angelicin |
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