| 复方首乌藤合剂的薄层鉴别和总黄酮的含量测定 |
| 投稿时间：2015-02-05 修订日期：2015-08-02 点此下载全文 |
| 引用本文：曾棋平,曹毅祥,黄丽珊,张荣.复方首乌藤合剂的薄层鉴别和总黄酮的含量测定[J].药学实践杂志,2016,34(5):424~427 |
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| 中文摘要:目的 建立复方首乌藤合剂的质控方法。方法 采用薄层色谱法对合剂中的首乌藤、合欢皮和五味子进行定性鉴别。以芦丁为对照品，采用紫外分光光度法测定总黄酮含量。结果 各供试品色谱中，在与对照品及对照药材色谱相应的位置上显相同颜色斑点，色谱清晰，附近无杂质斑点干扰。芦丁在9.12~27.36 μg/ml范围内与吸光度呈良好的线性关系，线性回归方程为A =0.034 55 C-0.110 34（r=0. 999 2），平均加样回收率为101.0%（RSD=1.58%）。结论 该方法操作简便、快速、有效、灵敏、准确、重现性好、专属性强，可作为复方首乌藤合剂的质量控制方法。 |
| 中文关键词:复方首乌藤合剂 薄层色谱法 总黄酮 紫外分光光度法 含量 |
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| TLC identification and determination of total flavonoids in Compound caulis Polygoni multiflori mixture |
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| Abstract:Objective To establish the quality control standards for Compound caulis Polygoni multiflori mixture. Methods Caulis polygoni multiflori, cortex alibiziae and schisand chinensis were identified by TLC, total flavonoids were determined by UV. Results Caulis polygoni multiflori,cortex alibiziae and schisand chinensis could be detected by TLC. A better linear relationship between concentration and absorbance in the range of 9.12~27.36 μg/ml. The linear regression equation was A=0.034 55 C-0.110 34 (r=0.999 2), The average recovery of emodin was 101.0%(RSD=1.58%). Conclusion The method is simple, quick, accurate and sensitive, which could be used as a quantitative analysis method for this preparation. |
| keywords:Compound caulis Polygoni multiflori mixture TLC total flavonoids ultraviolet spectrophometry content |
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