| HPLC-ELSD法同时测定小儿宝泰康颗粒中贝母素甲与贝母素乙的含量 |
| 投稿时间：2016-03-21 修订日期：2016-05-26 点此下载全文 |
| 引用本文：沈立茹,李瑞宏,王太亮.HPLC-ELSD法同时测定小儿宝泰康颗粒中贝母素甲与贝母素乙的含量[J].药学实践杂志,2016,34(4):351~353 |
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| 中文摘要:目的 建立HPLC-ELSD同时测定小儿宝泰康颗粒中2个指标成分贝母素甲、贝母素乙含量的方法。方法 采用Thermo C18（250 mm×4.6 mm，5μm）色谱柱，流动相：乙腈-水-二乙胺（65：35：0.03），流速：1.0 ml/min；ELSD参数为漂移管温度：85℃，载气流速：2.2 L/min。结果 贝母素甲在0.586～5.860μg （r=0.9996）、贝母素乙在0.564～5.640μg（r=0.9999）与峰面积的对数值呈线性关系；平均加样回收率分别为99.44%和98.56%，RSD分别为0.7%和1.0%。结论 该方法简便、准确、重复性好，可用于小儿宝泰康颗粒的质量控制。 |
| 中文关键词:小儿宝泰康颗粒 贝母素甲 贝母素乙 高效液相色谱-蒸发光散射检测法 |
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| Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD |
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| Abstract:Objective To develop a new high performance liquid chromatography (HPLC) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of two indicative components (peimine and peiminine) in Xiaoer Baotaikang granules. Methods The analysis was performed on a Thermo C18 column (250 mm×4.6 mm, 5 μm) by using a mobile phase consisted of acetonitrile-water and diethylamine(65:35:0.03), and the flow rate was 1.0 ml/min; ELSD parameters:the temperature of drift tube was 85℃ and the carrier gas flow rate was 2.2 L/min. Results The linear ranges of peimine and peiminine were 0.586~5.860 μg (r=0.9996) and 0.564~5.640 μg (r=0.9999), respectively. The average recovery rate for peimine and peiminine was 99.44% (RSD was 0.7%) and 98.56% (RSD was 1.0%),respectively. Conclusions The method is sample,accurate and reproducible, which could be used for the quality control of Xiaoer Baotaikang granules. |
| keywords:Xiaoer Baotaikang granules peimine peiminine HPLC-ELSD |
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