| 积雪草酸对颅脑损伤的保护作用及其机制研究 |
| 投稿时间：2016-03-18 修订日期：2016-04-01 点此下载全文 |
| 引用本文：顾申勇,朱秋珍,王静,刘玉兰,张悦,芮耀诚,章越凡,李铁军.积雪草酸对颅脑损伤的保护作用及其机制研究[J].药学实践杂志,2016,34(3):206~209,288 |
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| 基金项目:上海市青浦区卫生和计划生育委员会科研课题(W2015-02) |
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| 中文摘要:目的 研究积雪草酸对颅脑损伤的保护作用及其机制。方法 采用落重法建立大鼠闭合性颅脑损伤模型和液压冲击颅脑损伤模型,测定大鼠脑组织含水量、血脑屏障通透性、以及观察脑组织病理形态学变化,研究积雪草酸对颅脑损伤的保护作用;用酶联免疫吸附测定(ELISA)和聚合酶链式反应(PCR)检测炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的表达,探讨其作用机制。结果 积雪草酸可显著减少颅脑损伤后脑组织含水量,减轻血脑屏障通透性,改善脑组织细胞形态;并且可以显著抑制颅脑损伤后炎症因子TNF-α、IL-1β、IL-6的表达。结论 积雪草酸具有保护颅脑创伤的作用,其作用机制可能与其抑制炎症作用有关。 |
| 中文关键词:积雪草酸 颅脑损伤 炎症 |
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| Protective effects and mechanism of Asiatic Acid on traumatic brain injury |
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| Abstract:Objective To study the protective effects and mechanism of Asiatic Acid (AA) on traumatic brain injury. Methods The closed craniocerebral injury model made by the weight-drop method and fluid percussion brain injury model were used in the study. Brain water content, blood brain barrier permeability and brain tissue pathology morphology were measured to study the neuroprotective effect of AA on traumatic brain injury. Moreover, the inflammatory cytokines such as TNF-α, IL-β, IL-6 were examined by PCR and ELISA to study the mechanism of AA. Results Brain water content and permeability of blood brain barrier were significantly decreased, the traumatic brain injury was nameliorated by AA. Moreover, the expressions of inflammatory cytokines TNF-α, IL-β, IL-6 were inhibited by AA. Conclusion AA has the protective effects on traumatic brain injury and the mechanism is related to the anti-inflammatory effect. |
| keywords:Asiatic Acid traumatic brain injury inflammatory cytokines |
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