| 高效液相色谱法测定人血浆中阿克他利的浓度及其药动学研究 |
| 投稿时间：2014-03-25 修订日期：2015-02-01 点此下载全文 |
| 引用本文：何娟,周成杰.高效液相色谱法测定人血浆中阿克他利的浓度及其药动学研究[J].药学实践杂志,2016,34(1):41~43,75 |
| 摘要点击次数: 1428 |
| 全文下载次数: 104 |
|
|
| 中文摘要:目的 建立人血浆中阿克他利浓度的高效液相色谱检测方法。 方法 血浆样品采用液-液萃取的方法,采用Dikma C18柱分离,以水(含1.2%乙酸)-甲醇 (50:50, V/V) 的混合溶液为流动相,检测波长为245 nm,流速为1.0 ml/min,柱温30 ℃。 结果 建立的人血浆内阿克他利测定方法线性范围为75~4 000 ng/ml,定量下限可达75 ng/ml。日内、日间精密度(RSD)均小于10%,方法准确度大于90%,提取回收率大于88%。 结论 该方法快速、灵敏、专属,适用于人血浆中阿克他利浓度的测定。 |
| 中文关键词:阿克他利 高效液相色谱法 药动学 |
| |
| Study on pharmacokinetics and determination of actarit in human plasma by HPLC |
|
|
| Abstract:Objective To develop a simple and sensitive HPLC-UV method to determined actarit in plasma. Methods The chromatographic separation was carried out on a Dikma C18 column with a mobile phase composed of water containing 1.2% acetic acid-methanol (50:50, V/V) at a flow rate of 1.0 ml/min and the temperature of column was 30℃. UV wavelength mode was monitored at 245 nm. Results A linear response function was established for the range of concentrations 75-4 000 ng/ml for actarit. The inter-and intra-day precision value were less than 10% for actarit and the accuracy was more than 90%. The extraction recovery was more than 88%. Conclusion This simple, sensitive, and exclusive method could be used in routine clinical practice to monitor actarit concentrations in human plasma. |
| keywords:actarit HPLC pharmacokinetics |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
