| 应用高效液相色谱法测定消亢胶囊中迷迭香酸的含量 |
| 投稿时间：2014-11-25 修订日期：2015-04-03 点此下载全文 |
| 引用本文：肖金宝,姜宗文,孙丹,肖秋生,夏智.应用高效液相色谱法测定消亢胶囊中迷迭香酸的含量[J].药学实践杂志,2015,33(6):555~556,560 |
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| 基金项目:军队医疗机构制剂标准提高科研专项课题(No.14ZJZ03-2) |
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| 中文摘要:目的 建立测定消亢胶囊含量的方法。 方法 采用高效液相色谱(HPLC)法测定消亢胶囊中迷迭香酸的含量。色谱柱:Agilent C18柱,流动相:甲醇-0.5% 甲酸(50:50),流速:1 ml/min,检测波长:330 nm,柱温:30 ℃,进样量:10 μl。 结果 消亢胶囊中夏枯草迷迭香酸的浓度在2.88~20.16 μg范围内与峰面积积分值呈良好的线性关系(r=0.999 8),平均回收率为99.54%,RSD为0.40%(n=9)。 结论 该方法操作简便、结果准确可靠,适用于消亢胶囊中迷迭香酸的含量测定。 |
| 中文关键词:消亢胶囊 迷迭香酸 夏枯草 高效液相色谱 |
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| Determination of the rosmarinic acid in Xiaokang capsule by HPLC |
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| Abstract:Objective To develop a method for determination of the rosmarinic acid in Xiaokang capsules. Methods An HPLC method was developed. The determination was performed on Agilent C18 column at 30℃ with a mobile phase composed of methanol: 0.5% formic acid (50:50) run at a flow rate of 1.0 ml/min. The detection wavelength was set at 330 nm and the injection volume was 10 μl. Results The linear range of rosmarinic acid concentrations was 2.88 to 20.1 μg/ml (r=0.999 8). The average recovery was 99.5% (RSD=0.40%,r=9). Conclusion An assay for the determination of the rosmarinic acid in Xiaokang capsule that is simple, rapid, accurate and reliable has been developed. |
| keywords:Xiaokang capsule rosmarinic acid Prunella HPLC |
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