基于整体柱的表面增强拉曼光谱法检测非法染色青黛

郑滨兴; 吕迪亚; 方芳; 刘艳华; 李丹; 陆峰; 许激扬

基于整体柱的表面增强拉曼光谱法检测非法染色青黛

投稿时间：2015-02-09 修订日期：2015-05-28 点此下载全文

引用本文：郑滨兴,吕迪亚,方芳,刘艳华,李丹,陆峰,许激扬.基于整体柱的表面增强拉曼光谱法检测非法染色青黛[J].药学实践杂志,2015,33(5):426~428,459

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作者	单位	E-mail
郑滨兴	中国药科大学生命科学与技术学院, 江苏南京 210009
吕迪亚	第二军医大学药学院, 上海 200433
方芳	第二军医大学药学院, 上海 200433
刘艳华	中国药科大学生命科学与技术学院, 江苏南京 210009
李丹	福建中医药大学药学院, 福建福州 350108
陆峰	第二军医大学药学院, 上海 200433
许激扬	中国药科大学生命科学与技术学院, 江苏南京 210009	jiyangx@126.com

中文摘要:目的建立基于整体柱的表面增强拉曼光谱(surface enhanced Raman spectroscopy, SERS)法快速检测非法染色的青黛。方法采用混合溶剂乙醇-水(1:1,V/V)对染色青黛中的染料进行提取,利用整体柱中网状孔隙有助于银纳米颗粒在空间上耦合的特性,将样品溶液和银胶溶液混匀后滴加于整体柱上进行SERS光谱采集。结果此法可得到青黛中染色掺伪量低至500 μg/kg的孔雀石绿SERS信号。结论该方法具有快速、简便、专属性强等优点,可运用于中药材青黛非法染色的快速检测。

中文关键词:表面增强拉曼光谱整体柱非法染色青黛孔雀石绿

Detection of malachite green in natural indigo by surface enhanced Raman spectroscopy based on monolithic column

Abstract:Objective To develop a rapid SERS detection method based on monolithic column for detection of dye adulterated natural indigo. Methods The dyes in natural indigo were extracted and mixed with silver colloid. The spectra were recorded after applying the mixture solution to the monolithic column since the intertwined pores in monolithic column could contribute for the distribution of silver nanoparticles. Results SERS signals of malachite green dyed natural indigo at quantity as low as 500 μg/kg could be obtained. Conclusion This simple,fast and specific SERS detection method based on monolithic column could be used for rapid detection of stained natural indigo.

keywords:surface enhanced Raman spectroscopy (SERS) monolithic column adulteration natural indigo malachite green

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