| 中国南海群海绵Agelas mauritiana的化学成分研究 |
| 投稿时间：2015-03-05 修订日期：2015-04-16 点此下载全文 |
| 引用本文：方凤凯,汤华,刘宝姝,庄春林,孙鹏,张文.中国南海群海绵Agelas mauritiana的化学成分研究[J].药学实践杂志,2015,33(3):242~245,274 |
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| 基金项目:科技部863项目(2013AA092902),科技部国际合作项目(2014DFG32640),上海市卫生系统优秀学科带头人计划(XBR2013111),上海市优秀学科带头人计划(15XD1504600) |
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| 中文摘要:目的 对采自南中国海海域的群海绵(Agelas mauritiana)的化学成分进行研究。方法 采用硅胶色谱柱、Sephadex LH-20凝胶色谱柱、高效液相色谱等色谱方法,对Agelas mauritiana正丁醇萃取物进行分离纯化;应用现代波谱技术对化合物进行化学结构鉴定;用MTT法对化合物进行体外人肺癌细胞株A549细胞生长抑制活性测试。结果 共分离得到8个化合物,分别鉴定为:agelasine A (1)、agelasine B (2)、epi-agelasine C (3)、(-)agelasine D (4)、agelasine E (5)、agelasine F (6)、(-)ageloxime D (7)、aurantiamide acetate (8)。体外活性筛选中,这些化合物对A549显示出不同程度的生长抑制活性,化合物1~3的活性与阳性对照阿霉素相近。结论 化合物 1、2、4、5、6、8为首次从该种海绵中分离得到。首次选用A549对化合物1~7的活性进行评价,化合物2、3的显著生长抑制活性为进一步深入研究提供了依据。 |
| 中文关键词:群海绵 二萜生物碱 aurantiamide acetate 结构鉴定 肿瘤生长抑制活性 |
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| Study on chemical constitunts of sponge Agelas mauritiana from the South China Sea |
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| Abstract:Objective To investigate the chemical constituents of marine sponge Agelas mauritiana collected from the South China Sea. Methods The n-butanol extract of marine sponge Agelas mauritiana was separated and purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The chemical structures of those obtained compounds were determined on the basis of spectroscopic analysis and comparison with reported data. The tumor cell growth inhibitory activity of these compounds towards human lung carcinoma cells A549 was tested. Results Eight compounds were isolated, including agelasine A (1), agelasine B (2), epi-agelasine C (3), (-)agelasine D (4), agelasine E (5), agelasine F (6), (-)ageloxime D (7) and aurantiamide acetate (8). These compounds displayed different level of tumor cell growth inhibitory activity towards cell A549 in vitro. Compounds 1-3 showed significant activity towards cell A549, being similar to that of the positive control of adriamycin. Conclusion Compounds 1, 2, 4, 5, 6, 8 were isolated for the first time from the sponge Agelas mauritiana. Cell A549 was selected for the first time for the activity evaluation of compounds 1~7.Significant inhibition activity of compounds 2、3 may hold as a basis for further research. |
| keywords:Agelas mauritiana diterpene alkaloid aurantiamide acetate structure identification tumor cell growth inhibitory activity |
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