| 气相色谱-质谱联用结合代谢组学方法研究不同极化状态下小胶质细胞的代谢差异 |
| 投稿时间：2014-12-02 修订日期：2015-03-17 点此下载全文 |
| 引用本文：王彦,俞仲望,陈思,李玲,朱臻宇.气相色谱-质谱联用结合代谢组学方法研究不同极化状态下小胶质细胞的代谢差异[J].药学实践杂志,2015,33(3):226~230 |
| 摘要点击次数: 2423 |
| 全文下载次数: 988 |
|
| 基金项目:国家自然科学基金(81273474,31100765) |
|
| 中文摘要:目的 运用代谢组学方法阐明经典激活型(M1型)、选择活化型(M2型)和静息态小胶质细胞的代谢差异。方法 将体外培养小鼠小胶质细胞系(BV2)细胞,分为M1组、M2组和静息态组,用实时荧光定量聚合酶链反应(qRT-PCR)检测特异性mRNA的表达差异以确定细胞极化状态,采用基于气相色谱-质谱联用(GC-MS)技术的代谢组学方法阐明代谢变化。结果 发现M1型与静息态细胞的差异代谢物15个,M2型与静息态细胞的差异代谢物15个。结论 通过代谢组学方法可以找到小胶质细胞极化的差异代谢物,并解释其可能的极化机制,为神经退行性疾病的防治提供了理论依据。 |
| 中文关键词:气相色谱-质谱联用 代谢组学 小胶质细胞 实时定量PCR |
| |
| Comparative analysis of different states of polarized BV2 cells by GC-MS combined with metabonomic technology |
|
|
| Abstract:Objective To analyze the different metabolites of the classical activated(M1), alternatively activated(M2) and resting BV2 cells by metabolomics method. Methods The mRNAs of several potential biomarkers were determined by real-time PCR analyses to confirm the state of BV2 cells. Static GC-MS combined with metabolomics technology was used to analyze the metabolic changes. Results There were 15 biomarkers identified between the M1 group and the resting group, and 15 biomarkers were found in the M2 group and the resting group. Conclusion The present study provides an effective way to reveal the mechanism of the polarization of BV2 cell, and it might provide a theoretical basis to prevent or treat the neurodegenerative diseases. |
| keywords:GC-MS metabonomic microglia cell RT-PCR |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
