| 用超高效液相色谱蒸发光散射检测桔梗流浸膏指纹图谱的研究 |
| 投稿时间：2014-01-04 修订日期：2014-07-18 点此下载全文 |
| 引用本文：笔雪艳,胡畔,张胜波,张清波.用超高效液相色谱蒸发光散射检测桔梗流浸膏指纹图谱的研究[J].药学实践杂志,2015,33(2):153~155 |
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| 基金项目:国家药品标准提高暨2015版药典科研项目(TS-P021) |
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| 中文摘要:目的 建立桔梗流浸膏的指纹图谱检测方法,以达到质量的均一和稳定。 方法 采用UPLC-ELSD法,乙腈-水梯度洗脱,色谱柱:ACQUITY UPLC BEH C18 (2.1 mm×100 mm,1.7 μm),柱温:30 ℃,流速:0.2 ml/min,在20 min内,得到11个分离度良好的色谱峰。 结果 采用中药色谱指纹图谱相似度软件计算,桔梗流浸膏样品相似度均大于0.90,符合指纹图谱要求。 结论 该方法经稳定性、重复性、精密度考察,是一种快速、准确的中药质量评价方法。 |
| 中文关键词:桔梗 流浸膏 超高效液相色谱 蒸发光散射检测 指纹图谱 |
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| Research on fingerprint analysis of platycodonis liquid extract by UPLC-ELSD |
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| Abstract:Objective To develop a stable method for fingerprint analysis of extractum platycodi liquidum to ensure its uniformity and stability. Methods UPLC-ELSD was applied to obtain a chromatogram with 11 well separated peaks, and the chromatographic condition was as follows: mobile phase was gradient elution by acetonitrile-water, column was ACQUITY UPLC BEH C18 (2.1×100 mm, 1.7 μm) with the column temperature of 30 ℃ and the flow rate of 0.2 ml/min. Results The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM. Similarities were all greater than 0.90, which could meet the requirement for chromatographic fingerprint of TCM. Conclusion The stability, repeatability and precision of the developed method were all validated, and the chromatographic fingerprint analysis was a rapid and accurate method for the quality evaluation of traditional Chinese medicine. |
| keywords:Platycodonis liquid extract UPLC ELSD fingerprint |
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