| 索拉菲尼与异硫氰酸4-(甲基亚磺酰)丁酯体外协同杀伤肝癌细胞实验研究 |
| 投稿时间：2014-09-28 修订日期：2014-12-26 点此下载全文 |
| 引用本文：何文婷,巩志荣,孙治国,王美平,高洁,钟延强,鲁莹.索拉菲尼与异硫氰酸4-(甲基亚磺酰)丁酯体外协同杀伤肝癌细胞实验研究[J].药学实践杂志,2015,33(2):138~142 |
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| 中文摘要:目的 探讨索拉菲尼(sorafenib,SO)联合异硫氰酸4-(甲基亚磺酰)丁酯(sulforaphane,SF)杀伤肝癌细胞HepG2的协同比例。 方法 采用CCK-8法探讨SO和SF对HepG2杀伤效果最强的协同比例,利用协同指数(CI)反映协同杀伤效应:CI>1.1为拮抗;0.9结果 CCK-8法显示:SO与SF在摩尔比为1:1、1:10、1:20时,为拮抗作用(CI>1.1);在10:1时,为相加作用(0.9P<0.01),早期和晚期凋亡率(21.71±6.06)%显著高于拮抗比例1:1的凋亡率(6.64±0.31)%(P<0.01)。 结论 SO和SF摩尔比为5:1时能达到协同杀伤HepG2肝癌细胞的效果。 |
| 中文关键词:肝癌 索拉菲尼 异硫氰酸4-(甲基亚磺酰)丁酯 协同杀伤 |
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| Synergic effects of sorafenib combined with sulforaphane against hepatocellular carcinoma cells in vitro |
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| Abstract:Objective To investigate the synergic ratio of sorafenib (SO) and sulforaphane (SF) against the hepatocellular carcinoma cell line HepG2 in vitro. Methods The synergic effect of SO combined with SF against HepG2 cells was determined by the CCK8 assay (the synergic effect was determined by combination index (CI) value: CI>1.1, antagonistic; 0.9Results The CCK-8 assay showed that SO combined with SF at the molar rate of 1:1,1:10 and 1:20 exhibited strong antagonism (CI>1.1), whereas 20:1,5:1 and 1:5 ratio resulted in synergistic activity (CI<0.9). Furthermore, 10:1 ratio acted as an additive effective (0.9P<0.01). The early and late apoptosis rate of HepG2 cells (21.71±6.06) of the group treated with SO combined with SF at 5:1 synergistic ratio was significantly higher than that of the group treated with SO combined with SF at 1:1 antagonistic ratio(6.64±0.311)(P<0.01). Conclusion SO combined with SF at the molar rate of 5:1 can synergistically inhibit the growth of hepatocellular carcinoma cell line HepG2 in vitro. |
| keywords:hepatocellular carcinoma sorafenib(SO) sulforaphane(SF) synergic effec |
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