| 高效液相色谱法测定克拉维丁搽剂中醋酸地塞米松含量 |
| 投稿时间：2014-01-24 修订日期：2014-05-23 点此下载全文 |
| 引用本文：常明泉,陈林,何秀丽,陈芳.高效液相色谱法测定克拉维丁搽剂中醋酸地塞米松含量[J].药学实践杂志,2015,33(1):55~57 |
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| 基金项目:湖北省重大科学技术鉴定成果(登记号:EK2012D150018000359);太和科研基金资助项目(2009D34) |
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| 中文摘要:目的 测定克拉维丁搽剂中醋酸地塞米松的含量。方法 用高效液相色谱法,色谱柱为C18柱(150 mm×4.6 mm,5 μm);流动相为甲醇-水-冰醋酸(80:20:0.5);检测波长为240 nm;进样量20 μl;柱温30℃;流速1 ml/min。结果 醋酸地塞米松在2.00 ~64.00 μg/ml 范围内线性关系良好,Y=0.405 2X+0.804 2(r=0.999 9),平均回收率为99.3%,RSD=0.93%(n=9)。结论 该法便捷,重现性好,适用于克拉维丁搽剂中醋酸地塞米松的含量测定。 |
| 中文关键词:克拉维丁搽剂 醋酸地塞米松 高效液相色谱法 |
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| Determination of dexamethasone acetate in Kelaweiding liniment by HPLC |
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| Abstract:Objective To establish the quantitative determination method of dexamethasone acetate in Kelaweiding liniment by HPLC. Methods Column:Diamonsil-C18(150 mm×4.6 mm,5 μm),mobile phase:methanol-water-glacialaceticacid (80:20:0.5),flow rate:1.0 ml/min,UV detection wavelength:240 nm,injection volume :20 μl,column temperature:30℃. Results The standard curve was linear within the range of 2.00-64.00 μg/ml,linear equation was Y=0.405 2X+0.804 2(r=0.999 9).The average recovery was 99.3% with RSD 0.93% (n=9). Conclusion This method was simple,stable and could be used to assay dexamethasone acetate in Kelaweiding liniment. |
| keywords:Kelaweiding liniment dexamethasone acetate HPLC |
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