| 高效液相色谱法测定乌金胶囊中毛蕊异黄酮的含量 |
| 投稿时间：2014-01-27 修订日期：2014-04-11 点此下载全文 |
| 引用本文：王慧,李洋,吕昉,凌水花,黄玉凤,张国庆.高效液相色谱法测定乌金胶囊中毛蕊异黄酮的含量[J].药学实践杂志,2015,33(1):53~54,57 |
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| 中文摘要:目的 建立高效液相色谱法测定乌金胶囊中毛蕊异黄酮含量。方法 色谱柱为ZORBA Bonus-RP (4.6 mm×260 mm,5 μm)分析柱,流动相乙腈-水(含0.1%甲酸)梯度洗脱,柱温25℃,检测波长为260 nm,流速1.0 ml/min,进样量10 μl。结果 毛蕊异黄酮在7.4~117.6 μg/ml(r=1.000)浓度范围内呈良好线性关系,其回归方程为Y= 34.002X-3.451 2,回收率为95.24% (RSD=2.10%)。6批乌金胶囊中异黄酮含量在9.26~23.14 μg/g之间。结论 该方法简便、快速、准确,可用于乌金胶囊的质量控制。 |
| 中文关键词:高效液相色谱法 毛蕊异黄酮 含量测定 乌金胶囊 |
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| Determination of calycosin contents in Wujin capsule by HPLC |
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| Abstract:Objective To explore method for determination of calycosin contents in Wujin capsule by HPLC. Methods The sample was studied on ZORBA Bonus-RP (4.6 mm×260 mm,5 μm) column,the mobile phase consisted of acetonitrile and H2O-0.1% HCOOH (gradient elution),the temperature of column was 25℃,the UV detection wavelength was set at 260 nm,flow speed was 1.0 ml/min and injection volume was 10 μl. Results The calycosin calibration curve was at the range of 7.4-117.6 μg/ml (r=1.000),with their regression function being Y=34.002 X-3.451 2.The recovery of calycosin was 95.24% (RSD=2.10%).6 batches of Wujin capsule calycosin contents were 9.26-23.14 μg/g. Conclusion HPLC was an accuracy and simple method for determining the content of calycosin in Wujin capsule. |
| keywords:HPLC calycosin determination Wujin capsule |
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