| 双配体修饰聚合物泡囊的制备及细胞摄取 |
| 投稿时间：2014-10-11 修订日期：2014-11-25 点此下载全文 |
| 引用本文：解方园,俞媛,耿雯倩,钟延强.双配体修饰聚合物泡囊的制备及细胞摄取[J].药学实践杂志,2015,33(1):44~48 |
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| 基金项目:国家自然科学基金(81302714);上海市科委纳米专项(11nm0504400) |
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| 中文摘要:目的 构建一种主动靶向的新型纳米药物载体——聚合物泡囊(polymer vesicles,PVs),并考察其细胞摄取。方法 以马来酰亚胺-聚乙二醇-聚乳酸-羟基乙酸共聚物(MAL-PEG-PLGA)为载体材料,通过自组装制备PVs,用转铁蛋白(Tf)与Tet-1对PVs进行修饰,构建纳米药物载体(Tf/Tet-1-PVs)。以香豆素-6作为荧光探针包载于药物载体,考察脑微血管内皮细胞(BCEC)及神经细胞(Neuro-2a)对载体系统的摄取。结果 PVs粒径约80 nm,形态圆整,电镜观察具有明显膜层结构。BCEC细胞和Neuro-2a细胞对Tf/Tet-1-PVs的摄取均显著优于空白对照组和单配体修饰对照组。结论 PVs经双配体Tf及Tet-1修饰后可促进脑微血管内皮细胞和神经细胞的摄取。 |
| 中文关键词:脑靶向 聚合物泡囊 血脑屏障 |
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| The preparation and the cell uptake of polymer vesicles modified with dual ligands |
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| Abstract:Objective To construct an active targeting drug delivery system- polymer vesicles(PVs),and examined the cellular uptake. Methods Maleimide-polyethylene glycol-poly(lactic-co-glycolic acid)(MAL-PEG-PLGA)was used as carrier materials to prepare PVs by self-assembling.And then PVs was modified by Tf and Tet-1(Tf/Tet-1-PVs).To evaluate its active targeting,coumarin-6 was used as a fluorescent probe to analyze cellular uptake of PVs for both BCEC and Neuro-2a cells. Results PVs was about 80 nm with rounded shape and had obvious film structure.Tf/Tet-1-PVs exhibited a significant role in promoting cellular uptake for both BCEC and Neuro-2a cells compared with control and single ligand-modified group. Conclusion PVs modified with dual ligands could promote the cell uptake for both brain capillary cells and nerve cells. |
| keywords:brain targeting polymer vesicles blood-brain barrier(BBB) |
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