| 微小RNA传递载体聚乙二醇-b-聚赖氨酸的合成及细胞毒性研究 |
| 投稿时间：2014-02-21 修订日期：2014-06-28 点此下载全文 |
| 引用本文：任海峰,赵英魁,杨峰,俞媛,马志强.微小RNA传递载体聚乙二醇-b-聚赖氨酸的合成及细胞毒性研究[J].药学实践杂志,2014,32(5):362~365,373 |
| 摘要点击次数: 990 |
| 全文下载次数: 611 |
|
|
| 中文摘要:目的 制备微小RNA(miRNA)传递载体聚乙二醇-b-聚赖氨酸(PEG-b-PLL),并且选用模型细胞对其进行毒性考察。方法 通过核磁谱测定聚赖氨酸的聚合度,用4%琼脂糖凝胶电泳检测聚复合物对miRNA的包封,用动态光散射法测定制备所得聚复合物粒径、多分散性指数(PDI),Zeta电位,用荧光标记的FAM-hsa-miR-15a进行包封率的测定,采用CKK-8试剂盒测定K562细胞(人慢性淋巴白血病细胞)活力考察PEG-b-PLL的细胞毒性。结果 用PEG-b-PLL制备的聚阳离子胶束/miRNA复合物特性符合应用要求,并且细胞毒性显著低于聚乙烯亚胺(PEI),lipo2000。结论 离子聚合物载体PEG-b-PLL符合应用要求,同时毒性较低,有望成为基因转染的有效载体。 |
| 中文关键词:非病毒载体 聚赖氨酸 微RNA(miRNA) 细胞毒性 |
| |
| Study on synthesis and cyto-toxicity of polyethylene glycol-b-polylysine as potencial miRNA carrier |
|
|
| Abstract:Objective To synthesize miRNA carrier PEG-b-PLL and to testify the stability, encapsulation efficiency and cytotoxicity of its complexes.Methods H1NMR was used to determine the degree of polymerization of PLL, 4% agarose gel electrophoresis was used to determine entrapment of the polyer to the miRNA; then dynamic light scattering(DLS) was used to measure the hydrodynamic parameter such as size, polydispersity index(PDI) and zeta potential of the polyplexes. The entrapment efficiency was determined by ultraviolet-visible spectrophotometer, and finally, the cyto-toxicity of PEG-b-PLL was evaluated by CKK-8 kit with K562 cell lines.Results The characteristics indicated polyplexes prepared by PEG-b-PLL and miRNA fulfill the demand of being the gene carrier of miRNA because of low cyto-toxicity, high encapsulation efficiency and stability.Conclusion The miRNA carrier PEG-b-PLL had good character and low cyto-toxicity. It showed considerable potential as an efficient miRNA carrier. |
| keywords:non-viral vector polyethylenimine miRNA cyto-toxicity |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
