| 蒽酮染色法测定维地红涂剂中多糖的含量 |
| 投稿时间：2013-10-12 修订日期：2013-12-27 点此下载全文 |
| 引用本文：余学英,常明泉,陈芳,王刚.蒽酮染色法测定维地红涂剂中多糖的含量[J].药学实践杂志,2014,32(5):352~353,356 |
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| 基金项目:太和科研基金资助项目(2009D34). |
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| 中文摘要:目的 建立维地红涂剂中多糖的含量测定方法。方法 用无水葡萄糖为对照品,0.2%蒽酮-硫酸试液为染色剂,紫外分光光度法测定,测定波长620 nm。结果 多糖在1.9~34.2 μg/ml范围内线性关系良好,Y=0.033X-0.006 7(r=0.999 9),回收率为98.6%,RSD=1.06%(n=6)。结论 所建方法便捷、重现性好,适用于维地红涂剂中多糖的含量测定。 |
| 中文关键词:维地红涂剂 多糖 含量测定 |
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| Determination of the polysaccharide in Verdihong paint |
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| Abstract:Objective To establish the determination method of the polysaccharide in Verdihong paint.Methods The anhydrous dextrose was used as the control, 0.2% anthrone-sulfuric acid as the stain, the content of polysaccharide was determined by ultraviolet spectrophotometer at the wavelength of 620 nm.Results The polysaccharide curve was linear within a range of 1.9~34.2 μg/ml, linear equation was Y=0.033 X-0.006 7 (r=0.999 9), the average recovery was 98.6% with RSD of 1.06% (n=6).Conclusion This method was convenient, stable and coud be used to assay content of the polysaccharide in Verdihong paint. |
| keywords:Verdihong paint polysaccharide content determination |
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