| 牡丹皮化学成分的HPLC-QTOFMS分析 |
| 投稿时间：2013-07-05 修订日期：2014-05-14 点此下载全文 |
| 引用本文：赵文军,林阳,李鹏飞,刘洋.牡丹皮化学成分的HPLC-QTOFMS分析[J].药学实践杂志,2014,32(4):261~265 |
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| 中文摘要:目的 通过高效液相-单重四级杆-飞行时间质谱(HPLC-QTOFMS)联用技术,对中药牡丹皮的主要化学成分进行有效的鉴定。方法 色谱分离采用CAPCELL PAK C18(3.0 mm×100 mm, 3 μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.5 ml/min;质谱定性采用飞行时间质谱,正负离子模式扫描。结果 在优化的液质联用条件下,通过单重四级杆-飞行时间质谱鉴定牡丹皮中48个成分。结论 通过HPLC-QTOFMS联用技术,为鉴定牡丹皮中的化学成分建立起了一种快速、高效的分析方法。 |
| 中文关键词:牡丹皮 超高效液相-单重四级杆-飞行时间质谱 化学成分 |
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| Analysis of chemical constituents of Moutan cortex by HPLC-QTOFMS |
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| Abstract:Objective To analyze the main chemical constituents of Moutan cortex by high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-QTOFMS).Methods A CAPCELL PAK C18(3.0 mm×100 mm, 3 μm) column was used to do chromatographic separation.0.1% aqueous formic acid solution and acetonitrile were used as mobile phase composition.Gradient elution was used with a flow rate of 0.5 ml/min; QTOFMS was applied for qualitative analysis under positive and negative ion mode.Results 48 major constituents of Moutan cortex were identified by QTOFMS and structure-relevant fragment ions under the optimized condition.Conclusion A simple and reliable method with the usage of HPLC-QTOFMS was established to identify the main chemical constituents of Moutan cortex. |
| keywords:Moutan cortex HPLC-QTOFMS chemical constituents |
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