| 麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选 |
| 投稿时间：2014-01-15 修订日期：2014-04-02 点此下载全文 |
| 引用本文：韩琳,吕超,李敏,黄慧梅,畅婉琳,彭成成,柳润辉.麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选[J].药学实践杂志,2014,32(3):209~211,219 |
| 摘要点击次数: 1031 |
| 全文下载次数: 292 |
|
|
| 中文摘要:目的 建立原代心肌细胞缺氧-复氧模型,并对麝香保心丸及其20种血中活性成分进行抗缺氧-复氧损伤的活性筛选。方法 取新生(1~3 d)SD乳鼠的心脏,建立原代心肌细胞的缺氧-复氧损伤模型,并采用四甲基偶氮唑盐(MTT)比色法进行麝香保心丸及其入血成分的活性筛选。结果 麝香保心丸在50 μg/ml浓度下具有较好的保护原代心肌细胞缺氧-复氧损伤的作用;人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮具有较好的保护原代心肌细胞缺氧-复氧损伤的作用。结论 麝香保心丸具有抗心肌细胞缺氧-复氧损伤的作用,人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮是其主要的有效成分。该研究为麝香保心丸深入的药效和机制研究奠定了基础。 |
| 中文关键词:麝香保心丸 心肌细胞 缺氧-复氧模型 活性成分筛选 |
| |
| Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill |
|
|
| Abstract:Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill (SBP) absorbed in blood against hypoxia/reoxygenation injury.Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats(1~3 days old) and were used to build hypoxia/reoxygenation injury model. The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia/reoxygenation injury atthe concentration of 50 μg/ml. Ginsenoside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect against cardiomyocytes hypoxia/reoxygenation injury.Conclusion SBP have the protective activity against cardiomyocytes hypoxia/reoxygenation injury, and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP. This experiment offered basis for further pharmacodynamics and mechanism study of SBP. |
| keywords:Shexiang Baoxin Pill (SBP) cardiomyocyte hypoxia/reoxygenation model screening of active components |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
