| 用高效液相色谱法测定安坤颗粒中栀子苷和芍药苷的含量 |
| 投稿时间：2013-09-05 修订日期：2013-11-07 点此下载全文 |
| 引用本文：张丽萍.用高效液相色谱法测定安坤颗粒中栀子苷和芍药苷的含量[J].药学实践杂志,2014,32(2):121~123 |
| 摘要点击次数: 981 |
| 全文下载次数: 85 |
|
|
| 中文摘要:目的 建立HPLC法同时测定安坤颗粒中栀子苷和芍药苷的含量。方法 采用安捷伦Eclipse XDB C18色谱柱(250 mm×4.6 mm,5 μm),流动相:乙腈-水(14:86),检测波长:230 nm,柱温:35℃。结果 栀子苷在0.071 52~0.357 6 μg范围内线性关系良好(r=0.999 8),平均回收率为98.22%,RSD=1.25%。芍药苷在0.0719 7~0.359 85 μg范围内线性关系良好(r=0.999 2), 平均回收率为98.40%,RSD=0.68%。结论 该方法操作简便,结果准确,重复性好,可用于安坤颗粒的质量控制。 |
| 中文关键词:安坤颗粒 高效液相色谱法 栀子苷 芍药苷 |
| |
| Determination of gardenoside and paeoniflorin in Ankun granule by HPLC |
|
|
| Abstract:Objective To establish the HPLC method for the determination of gardenoside and paeoniflorin in Ankun granule. Methods The column was Agilent Eclipse XDB C18 (250 mm×4.6 mm,5 μm),and the acetonitrile-water(14:86) was used as the mobile phase.The detective wavelength was set at 230 nm. Results For the gardenoside,the linear range was 0.0715 2-0.357 6 μg,r=0.999 8 and the average recovery was 98.22% with RSD of 1.25%.For the Paeoniflorin,the linear range was 0.071 97-0.359 85 μg,r=0.999 2 and the average recovery was 98.40% with RSD of 0.68%. Conclusion The method was simple,reliable,and accurate which could be applied to the quality control of the drug. |
| keywords:Ankun granule HPLC gardenoside paeoniflorin |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
