| 高效液相色谱法同时测定扁咽口服液中黄芩苷和肉桂酸的含量 |
| 投稿时间：2012-12-17 修订日期：2013-04-08 点此下载全文 |
| 引用本文：马建丽,刘皈阳,李翔,黄欣欣,姚飞,马晓晖.高效液相色谱法同时测定扁咽口服液中黄芩苷和肉桂酸的含量[J].药学实践杂志,2013,31(6):451~453 |
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| 基金项目:解放军总医院第304临床部科研课题(YJ200922). |
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| 中文摘要:目的 建立高效液相色谱法同时测定扁咽口服液中黄芩苷和肉桂酸的含量。方法 色谱柱:Agilent Eclipse XDB-C18(4.6 mm×150 mm,5 μm);流动相:乙腈-2%冰醋酸溶液(23:77);流速:1.0 ml/min;检测波长:280 nm;柱温:室温;进样量:10 μl。结果 黄芩苷和肉桂酸分别在0.085 1~3.404 8 g(r=0.999 9)和0.020 3~0.811 2 g(r=0.999 9)范围内与其峰面积线性关系良好(r=0.999 9),平均回收率(n=6)为101.68%和101.46%,RSD为285%和2.07%。结论 本方法快速、简便、准确,能更全面控制和评价扁咽口服液的质量。 |
| 中文关键词:扁咽口服液 黄芩苷 肉桂酸 高效液相色谱法 含量测定 |
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| Determination of baicalin and cinnamic acid in Bianyan oral solution by HPLC |
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| Abstract:Objective To establish an HPLC method for determination of baicalin and cinnamic acid in Bianyan oral solution simultaneously. Methods The Agilent Eclipse XDB-C18(4.6 mm×150 mm, 5 μm)was used, the mobile phase consisted of ACN-2% acetic acid (23:77), the flow rate was 1.0 ml/min, the detection wavelength was at 280 nm, the column temperature was at room temperature, and the inject volume was 10 μl.Results A good linear correlation was observed within the range of 0.085 1~3.404 8 g for baicalin (r=0.999 9) and 0.020 3~0.811 2 g for cinnamic acid(r=0.999 9). The average recovery (n=6) was 101.68% with RSD 2.85% for baicalin and 101.46% with RSD 2.07% for cinnamic acid.Conclusion The method was rapid, simple and accurate, which could be used for the overall quality control of Bianyan oral solution. |
| keywords:Bianyan oral solution baicalin cinnamic acid HPLC determination |
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