| 高效液相色谱法测定养血安神丸中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量 |
| 投稿时间：2012-07-11 修订日期：2012-12-10 点此下载全文 |
| 引用本文：罗晨曲,吕情花.高效液相色谱法测定养血安神丸中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量[J].药学实践杂志,2013,31(5):386~387 |
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| 中文摘要:目的 建立HPLC法测定养血安神丸中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。方法 用Waters C18(4.6 mm×250 mm,5 μm)色谱柱,流动相:乙腈-水(20:80),流速:1.00 ml/min,检测波长:320 nm。结果 2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.086 7~1.083 5 μg范围内与峰面积线性关系好,r=0.999 8,平均回收率为99.2%,RSD为1.1%(n=9)。结论 本法测定简便,结果准确,灵敏度高,重复性好,可用于养血安神丸的质量控制。 |
| 中文关键词:高效液相色谱法 养血安神丸 2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 含量测定 |
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| Determination of 2,3,5,4'-stilbene glucoside in Yangxueanshen Pills by HPLC |
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| Abstract:Objective To establish a HPLC method for determination of stilbene glucoside in Yangxueanshen Pills. Methods The Waters C18 column (4.6 mm×250 mm, 5 μm) was used. The mobile phase was acetonitrile-water (20:80).The flow rate was 1.0 ml/min.The detection wavelength was 320 nm. Results The linear range was 0.086 7 μg~1.083 5 μg(r=0.999 8). The average recovery was 99.2% with RSD=1.1% (n=9). Conclusion The determination method was simple, accurate, sensitive and reproducible which could be used for quality control of Yangxueanshen Pills. |
| keywords:HPLC Yangxueanshen Pills 2,3,5,4'-stilbene glucoside determination |
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