| HPLC法测定二维亚铁颗粒中维生素C和维生素B1的含量 |
| 投稿时间：2012-07-27 修订日期：2013-01-21 点此下载全文 |
| 引用本文：李焕宇,邓朝晖.HPLC法测定二维亚铁颗粒中维生素C和维生素B1的含量[J].药学实践杂志,2013,31(5):380~383 |
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| 中文摘要:目的 建立同时测定二维亚铁颗粒中维生素C和维生素B1含量的HPLC方法。方法 采用Insteril ODS-3色谱柱,流动相为0.03 mol/L磷酸氢二铵溶液(加入0.01%庚烷磺酸钠,用磷酸调节pH至3.0)-甲醇,梯度洗脱,检测波长为244 nm,流速为0.6~1.0 ml/min,柱温30℃。结果 维生素C与维生素B1分别在0.501~6.011 μg(r=1.000 0)与 0.038~0.456 μg(r=0.999 9)范围内线性关系良好,平均回收率分别为99. 51%与99.20%,RSD分别为0.55%与0.49%(n=9)。结论 本法简便、结果准确可靠,可用于二维亚铁颗粒中维生素C和维生素B1的质量控制。 |
| 中文关键词:二维亚铁颗粒 维生素C 维生素B1 含量测定 高效液相色谱法 |
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| Quantitative determination of vitamin C and vitamin B1 in ferrous fumarate granules by HPLC |
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| Abstract:Objective To establish a method for the simultaneous determination of vitamin C and vitamin B1 in ferrous fumarate granules.Methods The procedure was performed on the Insteril ODS-3 C18 column at 30℃, detected at 244 nm. The mobile phase was composed by a gradient of 0.03 mol/L of ammonium phosphate solution (in which 0.01% of sodium heptanesulfonate was added and the pH was adjusted to 3.0 by phosphoric acid) and methanol, with the flux at 0.6~1.0 ml/min. Results Either vitamin C or vitamin B1 was in good linear at 0.501~6.011 μg, r=1.0000 and 0.038~0.456 μg, r=0.999 9, respectively. The mean recoveries were 99.51% and 99.20%, with the RSD 0.55% and 0.49%, n=9. Conclusion The method was proved to be simple, accurate and reliable, appropriate for the quality control for vitamin C and vitamin B1 in ferrous fumarate granules. |
| keywords:ferrous fumarate granules vitamin C vitamin B1 content determination HPLC |
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