| 皮肤角质形成细胞中TRPV1受体激活后的蛋白质组差异分析 |
| 投稿时间：2013-06-07 修订日期：2013-07-10 点此下载全文 |
| 引用本文：黄瑾,王建,袁芳,胡文娟.皮肤角质形成细胞中TRPV1受体激活后的蛋白质组差异分析[J].药学实践杂志,2013,31(5):334~337 |
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| 基金项目:上海市自然科学基金(12ZR1438500);浦东新区卫生局基金(PW2013A-12). |
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| 中文摘要:目的 研究皮肤角质形成细胞中辣椒素受体TRPV1激活后细胞中蛋白质组学的变化,并鉴定差异蛋白质。方法 皮肤角质形成细胞株HaCaT细胞经辣椒素(CAP)刺激24 h后,提取蛋白样品,采用双向凝胶电泳技术分离HaCaT细胞总蛋白,用PDQuest软件分析对照组和CAP刺激组蛋白组图谱的表达差异点,再运用串联飞行时间质谱(MALDI-TOF-MS)鉴定差异蛋白。结果 CAP刺激HaCaT细胞后,磷酸甘油醛异构酶、烯醇化酶、6-磷酸葡萄糖酸内酯酶和组织蛋白酶D蛋白表达水平增高,角蛋白14和60S酸性核糖体蛋白P2表达量降低,这些蛋白可能在TRPV1激活后影响细胞功能的过程中有重要作用。结论 利用蛋白质组学技术,研究皮肤角质形成细胞株HaCaT细胞中TRPV1受体激活后蛋白表达差异,为研究TRPV1受体的作用机制,以及为研发针对性药物,提供新的线索和方向。 |
| 中文关键词:皮肤角质形成细胞 辣椒素受体 双向电泳 蛋白质组学 |
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| Differential proteomics analysis of human keratinocytes with transient receptor potential vanilloid-1 activated by capsaicin |
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| Abstract:Objective To evaluate the differential proteomics of human keratinocytes with transient receptor potential vanilloid-1(TRPV1) activated by capsaicin.Methods Human keratinocytes were cultured and treated by capsaicin. Two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) combined with MALDI-TOF/TOF identification were used to investigate the effect of TRPV1 activation on the proteome of keratinocytes. Results Compared with blank control, triosephosphate isomerase(TPI), enolase 1,6-phosphogluconolactonase, cathepsin D had higher expression in peritoneal macrophages of experimental endometriosis, while keratin 14 and 60 S acidic ribosomal protein P2 expressed lower.Conclusion It was revealed that there was a potential mechanism of TRPV1 activation in keratinocytes, which gave a new sight to find targets and screen drugs in skin disorders. |
| keywords:transient receptor potential vanilloid-1 human keratinocytes capsaicin two-dimensional polyaerylamide gel electrophoresis |
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